##gff-version 3
##sequence-region BBa_K1677013 1 2721
BBa_K1677013	.	engineered_region	1846	2721	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1677013	.	terminator	2681	2721	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1677013	.	polyA_site	2708	2721	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1677013	.	engineered_region	2681	2721	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1677013	.	stem_loop	2688	2707	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1677013	.	stop_codon	2714	2714	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1677013	.	CDS	1865	2584	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1677013	.	CDS	1865	2584	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1677013	.	terminator	2593	2672	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1677013	.	stem_loop	2604	2647	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1677013	.	engineered_region	2593	2672	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1677013	.	ribosome_entry_site	1846	1858	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1677013	.	engineered_region	1846	1858	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1677013	.	ribosome_entry_site	1846	1858	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1677013	.	ribosome_entry_site	1852	1855	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1677013	.	engineered_region	229	1837	.	+	0	ID=BBa_K299811;Name=BBa_K299811
BBa_K1677013	.	CDS	248	1837	.	+	0	ID=BBa_K177026;Name=BBa_K177026;Parent=BBa_K299811
BBa_K1677013	.	CDS	248	1837	.	+	0	ID=annotation2011266;Name=protein;Parent=BBa_K177026
BBa_K1677013	.	ribosome_entry_site	229	241	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K299811
BBa_K1677013	.	engineered_region	229	241	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1677013	.	ribosome_entry_site	229	241	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1677013	.	ribosome_entry_site	235	238	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1677013	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_K1677013	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1677013	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1677013	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1677013	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1677013	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1677013	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1677013	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1677013	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1677013	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1677013	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1677013
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtcacacagg
aaagtactagatgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaa
gcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtccta
agacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtatt
agtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggag
aaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtg
ctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttag
cattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgca
gtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatg
acgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgt
aaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgtt
aatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcag
aaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtac
caaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaa
aattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagact
tacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttctt
aaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaa
atcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggta
acgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgcc
aggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgat
gaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtg
tagataatccaatcgaataatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgca
acatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactt
tcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggt
gatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaat
acaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagaca
caacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttta
ccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttg
agtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata