LOCUS BBa_K1677025 3747 bp DNA linear 29-Nov-2024 DEFINITION pBAD/AraC inducible Invasin ACCESSION BBa_K1677025 VERSION BBa_K1677025.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 CDS 1240..4065 /label=protein CDS 1240..3747 /label=BBa_K177010 misc_feature 1219..3747 /label=BBa_K299810 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagagc tattgaggct ccgcacacag 1261 cggatacgcc gtagccgcag atgttggtat gtcgccggtg gtcatatcca tagtgacaaa 1321 atctgtactg gtctttttag tccaatagtt acccgatggc cactcagcgc tatcataggt 1381 tgccaaactt ccccactctc cccacagagt cccgtctggt gaacgcgaac cattactggc 1441 gcgtgcggac tcgatcagcg cagtaaaatc agtgcgctgg caatttcgac tcgcctcgac 1501 acttgattgc agtgacatgg tgccggagaa cacccacaaa ttaggtttga attgatatgt 1561 tgccgtataa ctcgggaatt ttttactttt tgccgtcaca gtgacggtgc taccataggt 1621 tttataggca atattgactt taccctgatt atcaaccggc gcactggccg cataggatga 1681 tgtccagtca tattgagtat tattcgccac atcgtcattc atcaccaatt ggaacgtggc 1741 tttattaaag gtagttttcg ggaagccttt atctgtggca aattgctcac cattcacctt 1801 aatgccggtt atcttcggta ctgggtacag ggtgattctt ttctgcaaca agtctagtga 1861 ttcccccccc acctgcggcg taatatcgac atctcctacg gaatttccca ccacactggc 1921 ggtatagttg tcagcatttt cggttaccga actaatagtt accggaacac cactttgtat 1981 aaattcaaga tctgttatcc cactgacaaa ctcattattt ttattacgcg gaacaaaggt 2041 gagtatcgac tgcatggagc catcggcaac aatatcagat ggagaaacgt taaaacttga 2101 gtttgtgaca tctggcggca gcacggcaaa tcgtacatca acagtagcct gttgcccctg 2161 aatatctaaa gtgacgactg acaccccaac agtgaagcta gttaatgcaa tgcttatcac 2221 accctgtgca tcggtttttt ttgtgacttt actgggaagc gcaccattac tggtggttat 2281 caccccctct tgctcggcaa ccggcgtact atcaatatta gtcactgtaa atttcaccgt 2341 gatgggagtc ctgccatcag caagcgcacc atcatcagtc acttccgagg taataacgaa 2401 ctgaggttgc tgcacaataa cggtcaacgt agctggatta gaactattgc cgtggttatc 2461 gatagccgtg gcgctgaggg tataggtatt gaccgccgct tcgctctcgc cggttttccc 2521 cacagtacga ctcgctggtg ccatcggttt ataaggcggt aagaccacat tgtaatctgt 2581 tgcactgacg ggtattaatt tgccgccggc agcaacccat tgcgcatcat tccagagaat 2641 acgttgtagt gcagattgag actgtatttg tgcactaacg gaataaacct gccccggcag 2701 gccggagagt acggcgggtg aaaatgccaa tttgatagta ttctgttttt ggtattccag 2761 aacaatattt ggattgcgct caaccagatt ataacggctc tcagccagta aacgagttcc 2821 ggccaccact gcggggctaa actgcgaacg aaaactttcg ccgaggcgat aatccatttg 2881 taagttccat tgtatttcat gctgccgact ttttcccatt cgttgatcga cccccagtgt 2941 aatgaagggg atcggcgtat aaataagccc tgtggtcacc gcataagggt tactttgcag 3001 gttatcttta ccaaataaag ccacccgctc accacggtac tgctcatatt ttaatttccc 3061 gcccaattgt ggcagcgcag gtaaatacgc tttgacgtga atgtcgcccc cgcttgccgg 3121 gcgctcatta tagtccgcga aatcacgaga ttgatgccaa ccattgaggc gaaaataacc 3181 attggccgat aattgtaaat aatcagtcca ggcttctgca cccacgccaa tacgatgatt 3241 gtgcccggtc atatcattgt catagaaagt gttaaagcca tacatccaac tttgttggaa 3301 ggtacgcacc ccagcgccga tattaagggt attgcgactg tctttatttc ttatacccaa 3361 ctgactaaaa aagacatatg aagcagagtc ataccaaggc aacagccaat caagagaact 3421 ttctttgagg gagaactttt tatcaaagtt aacattgacc tgagttgtcc caaatctatt 3481 taaccagtgt tttacttcct gatttgccgc gtcatttacc attgacctgg tgatattgga 3541 tgcattaccc gataccgcca gactggttcc ggtactgatt atcttattga aagcctcatt 3601 atgttcagcg gggttctcta acgcttctga attaaaataa tgttgctgtg agaacccata 3661 agtaaatatt ccaaatatta aaccgatcga taatattagc ctgctaatta ttttagtcac 3721 agttagcgta ttaaaaaatg aatacat //