BBa_K1677102 1 BBa_K1677102 Invasin with Usp45 Signal Peptide (Lactococcus) 2015-08-19T11:00:00Z 2015-08-20T02:16:31Z The invasin gene was originally source by the Warsaw 2010 team from the bacterium Yersinia something or other. Invasin enables bacteria to invade mammalian cells via beta-integrin mediated endocytosis. Invasin was codon optimised for lactococcus species, and the lactococcal Usp45 signal peptide was added to localise it to the outermembrane. false false _2095_ 25019 25019 9 false This part was assembled by overlap PCR of 5 IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis. false Mackenzie Labine-Romain BBa_K1677102_sequence 1 atgaagaagaagatcatttccgctattttgatgtcaacagttattttatcagctgctgctccattatcaggtgtttatgctatgatgacattacaaatccatacaggtggtattaatttaaagaagaagaatatttattcgattcgtaagttaggtgttggtattgcttcagttacattaggtacattattgatttcaggtggtgttacaccagctgctaatgctgctcaacatgatgaagctcaacaaaatgctttctatcaagttttgaacatgccaaatttgaatgctgatcagcgtaacggatttatccaatccttaaaggatgacccatcacaatccgctaatgtattaggtgaagctcagaagttgaatgactcacaagcgcctaaggctgatgctcagcaaaacaatttcaataaggaccaacaatcagcgttctacgaaatcttaaatatgccgaacttgaacgaggcacaacgcaatggattcattcaatcgttaaaagacgatccatctcaatcaacaaatgttttaggtgaagctaagaagttaaatgaatcacaagctccaaaggcagataataatttcaataaggagcaacagaatgctttttacgaaattttgaatatgccaaacttaaatgaagaacagcggaacgggtttatccaatcattgaaggacgacccatctcaatccgctaatttattggctgaggctaagaaattaaacgatgctcaggctcctaaggctgacaataagttcaataaggaacaacaaaacgctttctatgagatcttgcatttaccaaatttaacagaggaacagcgtaatggttttattcaatcattaaaggatgatccatcagtttcaaaggagatcttagccgaggcgaagaaattgaatgatgctcaggctccaaaggaagaggataacaataagccgggcaaggaggataataacaaaccaggcaaagaagacaacaacaagcctggtaaagaggacgggaacaagcccgggaaagaagataacaagaagccaggtaaggaagatggcaataaacctgggaaggaggacaataagaaaccggggaaggaagatggtaacaaacccggcaaggaggacggaaataaacccggaaaagaagatggcaatggtgttcatgttgttaagcccggtgatacagttaatgatattgctaaggctaatggtacaacagctgataagattgctgccgataataagttagcagataagaatatgatcaagccggggcaagaattagttgttgataagaagcaaccagctaatcatgctgatgctaataaggctcaagctttaccagaaacaggtgaagaaaatccatttattggtacaacagtttttggtggtttatcattagctttaggtgctgctttattagctggtcgtcgtcgtgaattagttcaacaaccacaattaacattaacagctgctgttattggtgatggtgctccagctaatggtaaaacagctattacagttgaatttacagttgctgattttgaaggtaaaccattagctggtcaagaagttgttattacaacaaataatggtgctttaccaaataagattacagaaaagacagatgctaatggtgttgctcgtattgctttaacaaatacaacagatggtgttacagttgttacagctgaagttgaaggtcaacgtcaatcagttgatacacattttgttaaaggtacaattgctgctgataaatcaacattagctgctgttccaacatcaattattgctgatggtttaatggcttcaacaattacattagaattaaaagatacatatggtgatccacaagctggtgctaatgttgcttttgatacaacattaggtaatatgggtgttattacagatcataatgatggtacatattcagctccattaacatcaacaacattaggtgttgctacagttacagttaaagttgatggtgctgctttttcagttccatcagttacagttaattttacagctgatccaattccagatgctggtcgttcatcatttacagtttcaacaccagatattttagctgatggtacaatgtcatcaacattatcatttgttccagttgataaaaatggtcattttatttcaggtatgcaaggtttatcatttacacaaaatggtgttccagtttcaatttcaccaattacagaacaaccagattcatatacagctacagttgttggtaatacagctggtgatgttacaattacaccacaagttgatacgttaatcttatcaacattacaaaagaagatctccttatttccagttccgacattaaccggtattttggttaatggccaaaatttcgccacagacaaaggttttccaaagacaatctttaagaatgcgacatttcaattacaaatggataatgatgtcgctaataatacacaatatgaatggtcatcatcatttacaccaaatgtttcagttaatgatcaaggtcaagtgacaattacatatcaaacatattccgaagttgctgtcacagctaaatcaaaaaaatttccatcgtattcagtttcatatcgtttttatccaaatcgttggatttatgatggtggtacatcattagtttcatcattagaagcttcacgtcaatgtcaaggttcagatatgtcagctgttttagaatcatcacgtgctacaaatggtacacgtgctccagatggtacattatggggtgaatggggttcattaacagcttattcatcagattggcaatcaggtgaatattgggttaaaaaaacatcaacagattttgaaacaatgaatatggatacaggtgctttagttcaaggtccagcttatttggctttcccattatgtgcgttagctatttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z