BBa_K1677391
1
BBa_K1677391
IBV Pseudoknot
2015-08-24T11:00:00Z
2015-08-25T01:40:08Z
From the infectious bronchitis virus.
This is part of the BABS iGEM pseudoknot suite. This pseudoknot can be used for delayed translation of a protein sequence, by slowing the ribosome.
false
false
_2095_
24918
24918
9
false
All start codons had to be removed from the pseudoknot. <br>
20 base pairs from the viral DNA flank either side of the pseudoknot region. <br>
3' flanking sequence AGAATTATTTAAACGGGTAC<br>
5' flanking sequence TTTGAGGTTTGTAATAAGGA<br>
The actual pseudoknot is: <br>
GGGGTAGCAGTGAGGCTCGGCTGATACCCCTTGCTAGTGGGTGTGACCCTGATATTGTAAAGCGAGCC <br>
((((((.((((..[[[[[[)))).))))))((((....((((...)))).......))))..]]]]]] <br>
Estimated free energy: -32.02 kcal/mol <br>
false
Isabelle Capell-Hattam
annotation2466068
1
IBV Pseudoknot structure
range2466068
1
21
87
annotation2466067
1
5' Flanking Region
range2466067
1
88
108
annotation2466066
1
3' Flanking Region
range2466066
1
1
20
BBa_K1677391_sequence
1
agaattatttaaacgggtacggggtagcagtgaggctcggctgataccccttgctagtgggtgtgaccctgatattgtaaagcgagcctttgaggtttgtaataagga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z