BBa_K1677391 1 BBa_K1677391 IBV Pseudoknot 2015-08-24T11:00:00Z 2015-08-25T01:40:08Z From the infectious bronchitis virus. This is part of the BABS iGEM pseudoknot suite. This pseudoknot can be used for delayed translation of a protein sequence, by slowing the ribosome. false false _2095_ 24918 24918 9 false All start codons had to be removed from the pseudoknot. <br> 20 base pairs from the viral DNA flank either side of the pseudoknot region. <br> 3' flanking sequence AGAATTATTTAAACGGGTAC<br> 5' flanking sequence TTTGAGGTTTGTAATAAGGA<br> The actual pseudoknot is: <br> GGGGTAGCAGTGAGGCTCGGCTGATACCCCTTGCTAGTGGGTGTGACCCTGATATTGTAAAGCGAGCC <br> ((((((.((((..[[[[[[)))).))))))((((....((((...)))).......))))..]]]]]] <br> Estimated free energy: -32.02 kcal/mol <br> false Isabelle Capell-Hattam annotation2466068 1 IBV Pseudoknot structure range2466068 1 21 87 annotation2466067 1 5' Flanking Region range2466067 1 88 108 annotation2466066 1 3' Flanking Region range2466066 1 1 20 BBa_K1677391_sequence 1 agaattatttaaacgggtacggggtagcagtgaggctcggctgataccccttgctagtgggtgtgaccctgatattgtaaagcgagcctttgaggtttgtaataagga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z