BBa_K1677666 1 BBa_K1677666 HCV229E Pseudoknot 2015-08-24T11:00:00Z 2015-08-25T02:21:08Z Hepatitis C virus 299E This is part of the BABS iGEM pseudoknot suite. This pseudoknot can be used for delayed translation of a protein sequence, by slowing the ribosome. false false _2095_ 24918 24918 9 false All start codons had to be removed from the pseudoknot. <br> 20 base pairs from the viral genome flank either side of the pseudoknot region. <br> 3' flanking sequence AACAGTTATTTAAACGAGTC <br> 5' flanking sequence AGGCTGTAGTGCTGTTGCTA <br> The actual pseudoknot is: <br> CGGGGCUCUAGUGCCGCUCGACUAGAGCCCUGUAUUGGUACAGACAUAGAUUACUGUGUCCGUGCAUUUGACGUUUACAAUAAAGAUCCGUCUUUUAUCGGAAAAAAUCUGAAGACCAAUUGUGUGUGCUUCAAGAAUAUAGAUAAGGUUGACGGGUUCUAUAUUGUUAAACGUUGCAAUAAGUCAGUUAAGGACCACGAGCAGUCCUUG <br> ((((((((((((...[[[[[))))))))))))(((((((((.(((((((....))))))).))))....(((((((((((((...(((((((((((((((((.....)))..............((((.........)))))))))))..)))))))....))))).)))))))).))))).......(((((((..]]]]].))))))) <br> Estimated free energy: -74.29 kcal/mol false Isabelle Capell-Hattam annotation2466089 1 3' Flanking Region range2466089 1 1 20 annotation2466092 1 HCV229E Pseudoknot range2466092 1 20 244 annotation2466091 1 5' Flanking Region range2466091 1 244 264 BBa_K1677666_sequence 1 aacagttatttaaacgagtccggggctctagtgccgctcgactagagccctgtattggtacagacatagattactgtgtccgtgcatttgacgtttacaataaagatccgtcttttatcggaaaaaatctgaagaccaattgtgtgtgcttcaagaatatagataaggttgacgggttctatattgttaaacgttgcaataagtcagttaaggaccacgagcagtccttgtataacttacttaaaggctgtagtgctgttgcta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z