BBa_K1677666
1
BBa_K1677666
HCV229E Pseudoknot
2015-08-24T11:00:00Z
2015-08-25T02:21:08Z
Hepatitis C virus 299E
This is part of the BABS iGEM pseudoknot suite. This pseudoknot can be used for delayed translation of a protein sequence, by slowing the ribosome.
false
false
_2095_
24918
24918
9
false
All start codons had to be removed from the pseudoknot. <br>
20 base pairs from the viral genome flank either side of the pseudoknot region. <br>
3' flanking sequence AACAGTTATTTAAACGAGTC <br>
5' flanking sequence AGGCTGTAGTGCTGTTGCTA <br>
The actual pseudoknot is: <br>
CGGGGCUCUAGUGCCGCUCGACUAGAGCCCUGUAUUGGUACAGACAUAGAUUACUGUGUCCGUGCAUUUGACGUUUACAAUAAAGAUCCGUCUUUUAUCGGAAAAAAUCUGAAGACCAAUUGUGUGUGCUUCAAGAAUAUAGAUAAGGUUGACGGGUUCUAUAUUGUUAAACGUUGCAAUAAGUCAGUUAAGGACCACGAGCAGUCCUUG <br>
((((((((((((...[[[[[))))))))))))(((((((((.(((((((....))))))).))))....(((((((((((((...(((((((((((((((((.....)))..............((((.........)))))))))))..)))))))....))))).)))))))).))))).......(((((((..]]]]].))))))) <br>
Estimated free energy: -74.29 kcal/mol
false
Isabelle Capell-Hattam
annotation2466089
1
3' Flanking Region
range2466089
1
1
20
annotation2466091
1
5' Flanking Region
range2466091
1
244
264
annotation2466092
1
HCV229E Pseudoknot
range2466092
1
20
244
BBa_K1677666_sequence
1
aacagttatttaaacgagtccggggctctagtgccgctcgactagagccctgtattggtacagacatagattactgtgtccgtgcatttgacgtttacaataaagatccgtcttttatcggaaaaaatctgaagaccaattgtgtgtgcttcaagaatatagataaggttgacgggttctatattgttaaacgttgcaataagtcagttaaggaccacgagcagtccttgtataacttacttaaaggctgtagtgctgttgcta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z