LOCUS       BBa_K1677800             336 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG inducible promoter with IBV pseudoknot
ACCESSION   BBa_K1677800
VERSION     BBa_K1677800.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..248
                     /label=3' Flanking Region
     misc_feature    316..336
                     /label=5' Flanking Region
     misc_feature    249..315
                     /label=IBV Pseudoknot structure 
     misc_feature    229..336
                     /label=BBa_K1677391
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagag aattatttaa
      241 acgggtacgg ggtagcagtg aggctcggct gatacccctt gctagtgggt gtgaccctga
      301 tattgtaaag cgagcctttg aggtttgtaa taagga
//