BBa_K1678001 1 BBa_K1678001 Pyrimidine reductase/pyrimidine deaminase cassette RibD 2015-09-15T11:00:00Z 2015-09-18T06:33:58Z Coding sequence comes from Bacillus subtilis genome sequence. It has been codon optimized for Lactobaciilus plantarum on IDT website. p25 is a synthetic constitutive promoter designed in 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006. The RBS informations are coming from Tauer et al. Microbial Cell Factories 2014, 13:150. Tldh terminator information are documented in Spath et al. Microbial Cell Factories 2012, 11:141. Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. Coding sequence has been codon optimized for Lactobacillus plantarum. The gene is promoted by the synthetic promoter p25 from 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006. The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses two steps in the riboflavin pathway. false false _2096_ 27599 25228 9 false RibD is not considered as a bottleneck in the riboflavin pathway. A medium promoter is so expressing it. false Barthelemy Caron annotation2474743 1 Tldh terminator range2474743 1 1181 1318 annotation2474738 1 Golden Gate assembly site range2474738 1 1 19 annotation2474742 1 RibD range2474742 1 95 1180 annotation2474741 1 RBS optimal spacing range2474741 1 87 94 annotation2474739 1 BBa_K1678004 range2474739 1 19 79 annotation2474744 1 Golden Gate assembly site range2474744 1 1319 1346 annotation2474740 1 RBS range2474740 1 80 86 BBa_K1678001_sequence 1 cgctatagaagacttgagaagatctgtggggttagttgttgacattaaggcacatcattgatatggtttaattatagcgaaggaggaaatttacatggaagaatattatatgaagttggctttggatttggcaaaacaaggcgaaggccaaactgaatcaaatccacttgttggcgctgtagtagtaaaagacggccagatcgttggcatgggcgctcacttgaagtatggtgaagcacacgctgaagtacatgctattcacatggctggcgcacacgctgaaggcgcagatatttatgttacattggaaccttgttctcattacggcaaaactccaccatgtgctgaattgattattaatagtggcattaagcgcgttttcgtagctatgcgcgatccaaatccattggttgctggccgcggcatttcaatgatgaaggaggcaggcatcgaagtacgcgaaggtattttggctgaccaagctgaacgcttgaatgaaaagttccttcattttatgagaactggccttccttatgttactcttaaggccgccgcctcattggacggcaagattgccacctcaaccggcgattctaagtggatcacctcagaagccgcccgtcaagatgcccaacaatatcgaaagacacaccaatcaattttggttggtgtaggcacagtaaaagccgacaatccatcattaacttgccgattaccaaacgttaccaagcaacctgtaagagtcattcttgataccgttttgtctattccagaggatgccaaggttatttgtgaccaaattgctcctacatggatttttaccactgcccgagccgatgaagaaaaaaaaaagcgtttgagtgccttcggcgtaaacatttttacacttgaaacagaacgcattcaaattccagatgttttgaagattttagccgaagaaggcatcatgagtgtatacgttgaaggcggctcagccgtacatggaagttttgtcaaggaaggctgttttcaagaaattattttctattttgccccaaaattgatcggcggcactcatgccccaagtttgatttcaggcgaaggctttcaatcaatgaaggacgttcctttgttgcaatttactgacattactcagattggccgcgatattaaattgacagccaagccaacaaaagaataagctcgtcaggttaagcgctcagcttaacggtgtctctccagacgagtatcactaagccaccgtaccaaaaaaccgctgtccgagaccgcgcgtcacaacgcgggcaatctcaggcagcggcttttttaatctttttctgcgcttgtcttcatatgtggtgccgcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z