BBa_K1678006
1
BBa_K1678006
mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus
2015-09-15T11:00:00Z
2016-01-25T01:10:59Z
The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006
The terminator comes from the following publication: 'Spath et al. Microbial Cell Factories 2012, 11:141'
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'
The whole cassette was artificially synthesized.
The gene cassette comprises of T48 lactobacillus promoter, TldH terminator, and mCerulean gene codon optimized for lactobacillus acidophilus (plantarum). It expresses mCerulean specific cyan fluorescence in E. coli as well.
false
false
_2096_
4206
27494
9
false
None
false
Prateek Garg
annotation2474429
1
RBS
range2474429
1
61
75
annotation2474430
1
terminator Tldh
range2474430
1
796
933
annotation2474428
1
BBa_K1678004
range2474428
1
1
60
annotation2462512
1
mCereulean
range2462512
1
76
795
BBa_K1678006_sequence
1
cgctatagatctgcatcgtaagttgttgacatggaacgaggaatgtgataatctgtgagtatagcgaaggaggaaatttacatggtaagtaaaggtgaagaattattcactggtgtagttcctattttagtagaattagacggtgatgtaaatggtcataaattttcagtatctggtgagggtgaaggtgacgctacttatggtaagttaactttgaagttcatttgtactactggcaagttaccagttccttggccaactcttgttactactttaacttggggtgtacaatgtttcgcacgttaccctgaccatatgaagcaacatgatttctttaagagcgcaatgcctgaaggttatgtacaagaacgtacaattttcttcaaggacgatggtaactacaaaactcgcgcagaagtcaaattcgagggcgacacacttgtaaatagaattgagttgaagggtatcgacttcaaggaggatggcaatattttgggccacaagttagaatacaatgcaatttcagacaacgtttatatcactgccgataagcagaagaacggcattaaggcaaacttcaaaattcgtcacaatattgaggacggcagtgttcagttagccgatcactaccaacaaaatactcctattggcgacggtccagttcttcttccagataaccactatttgtctacccagagtaagttgtcaaaagatccaaacgaaaagcgtgatcacatggtccttttggagtttgtaacagcagcaggtattactttaggaatggatgaattatataagtaagctcgtcaggttaagcgctcagcttaacggtgtctctccagacgagtatcactaagccaccgtaccaaaaaaccgctgtccgagaccgcgcgtcacaacgcgggcaatctcaggcagcggcttttttaatctttttct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z