LOCUS       BBa_K1678007            1252 bp    DNA     linear       26-Jan-2026
DEFINITION  Vitamin B12 biosensor, composed by a cobalamin riboswitch in front 
            of an mRFP1
ACCESSION   BBa_K1678007
VERSION     BBa_K1678007.1  
FEATURES             Location/Qualifiers
     promoter        137..168
                     /label=Lac Promoter
     misc_feature    171..193
                     /label=Lac O
     misc_feature    200..409
                     /label=Riboswitch sequence from Propionibacterium
     RBS             378..385
                     /label=Supposed RBS
     CDS             389..409
                     /label=Supposed CbiB truncated protein
     CDS             413..1087
                     /label=mRFP1 without start codon
     misc_feature    410..412
                     /label=Scar
     misc_feature    1135..1178
                     /label=rrnB T1 terminator
     misc_feature    249..305
                     /label=Predicted riboswitch (aptamer) by Rfam database
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacact gtactagggt caatgtgctg gcctcggcca gcgcgcgtcc
      241 gcagcgaagc cggtgggaat ccggcactgt cccgcaacgg tgatggggcc cggccccgag
      301 tccggtcgac tgtggacgtg tccccacatt gccccggcgc aaggtcagcc ccacgagctg
      361 cctgcgcgtg caccgcgaag gacggcccgt ggatttgagg ttcttgtccg aggcttcctc
      421 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      481 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      541 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      601 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      661 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      721 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      781 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      841 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      901 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      961 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1021 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1081 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1141 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1201 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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