LOCUS       BBa_K1679011             807 bp    DNA     linear       26-Dec-2024
DEFINITION  promoter+RNA thermometer+RFP
ACCESSION   BBa_K1679011
VERSION     BBa_K1679011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             90..95
                     /label=rbs
     misc_feature    66..71
                     /label=scar adaptation
     stem_loop       44..61
                     /label=stem_loop
     stem_loop       67..95
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    44..95
                     /label=BBa_K115002
     CDS             102..776
                     /label=mrfp1
     misc_feature    783..807
                     /label=Help:Barcodes
     CDS             102..807
                     /label=BBa_E1010
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagggacaag caatgcttgc
       61 cttgaatagt aacttttgaa tagtgattca ggaggtacta gatggcttcc tccgaagacg
      121 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      181 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      241 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      301 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
      361 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
      421 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
      481 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
      541 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      601 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      661 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      721 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      781 aacgctgata gtgctagtgt agatcgc
//