LOCUS       BBa_K1679016             851 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter+RNA thermometer (ROSE)+mRFP
ACCESSION   BBa_K1679016
VERSION     BBa_K1679016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             133..138
                     /label=SD
     misc_feature    117..122
                     /label=Biobrick-Scar-adaptation
     stem_loop       44..58
                     /label=Predicted Stem Loop
     stem_loop       59..79
                     /label=Predicted Stem Loop
     stem_loop       82..114
                     /label=Predicted Stem Loop
     stem_loop       115..139
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    44..139
                     /label=BBa_K115001
     CDS             146..820
                     /label=mrfp1
     misc_feature    827..851
                     /label=Help:Barcodes
     CDS             146..851
                     /label=BBa_E1010
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gaggccgcga caagcggtcc
       61 gggcgcccta ggggcccggc ggagacgggc gccggaggtg tccgacgcct gctcgtcaag
      121 ttcttgctcc ttggaggatt actagatggc ttcctccgaa gacgttatca aagagttcat
      181 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      241 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      301 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      361 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      421 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      481 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      541 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      601 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      661 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      721 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      781 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
      841 gtgtagatcg c
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