##gff-version 3
##sequence-region BBa_K1679019 1 1690
BBa_K1679019	.	CDS	985	1690	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1679019	.	engineered_tag	1666	1690	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1679019	.	CDS	985	1659	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1679019	.	promoter	884	918	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K1679019	.	engineered_region	1	875	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_K1679019	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_K1679019	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1679019	.	CDS	19	738	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_K1679019	.	CDS	19	738	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1679019	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_K1679019	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1679019	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1679019	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_K1679019	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1679019	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1679019	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1679019	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1679019	.	mature_transcript_region	927	978	.	+	0	ID=BBa_K115002;Name=BBa_K115002
BBa_K1679019	.	ribosome_entry_site	973	978	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K1679019	.	sequence_alteration	949	954	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K1679019	.	stem_loop	950	978	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K1679019	.	stem_loop	927	944	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
>BBa_K1679019
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagggacaagcaatgcttgccttga
atagtaacttttgaatagtgattcaggaggtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttca
aagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtac
ccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtac
ggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggg
aacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatcta
caaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcc
accgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcact
acgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaact
ggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct
taataacgctgatagtgctagtgtagatcgc