LOCUS       BBa_K1679021            1830 bp    DNA     linear       25-Nov-2024
DEFINITION  J23119+K1357010+J23119+I13504
ACCESSION   BBa_K1679021
VERSION     BBa_K1679021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..736
                     /label=mrfp1
     misc_feature    743..767
                     /label=Help:Barcodes
     CDS             62..767
                     /label=BBa_E1010
     stem_loop       787..830
                     /label=stem_loop
     misc_feature    776..855
                     /label=BBa_B0010
     terminator      776..855
                     /label=BBa_B0010
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     terminator      776..904
                     /label=BBa_B0015
     misc_feature    44..904
                     /label=BBa_K1357010
     promoter        913..947
                     /label=BBa_J23119
     misc_feature    960..963
                     /label=conserved
     RBS             956..967
                     /label=BBa_B0034
     CDS             974..1693
                     /label=GFP protein
     CDS             974..1693
                     /label=BBa_E0040
     stem_loop       1713..1756
                     /label=stem_loop
     misc_feature    1702..1781
                     /label=BBa_B0010
     terminator      1702..1781
                     /label=BBa_B0010
     stem_loop       1797..1816
                     /label=T7 TE
     misc_feature    1823..1823
                     /label=stop
     polyA_site      1817..1830
                     /label=polya
     misc_feature    1790..1830
                     /label=BBa_B0012
     terminator      1790..1830
                     /label=BBa_B0012
     misc_feature    956..1830
                     /label=BBa_I13504
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
      541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
      601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
      661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
      721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tatatactag agttgacagc tagctcagtc ctaggtataa tgctagctac tagagaaaga
      961 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1021 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1081 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1141 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1201 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1261 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1321 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1381 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1441 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1501 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1561 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1621 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1681 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1801 caccttcggg tgggcctttc tgcgtttata
//