LOCUS BBa_K1679021 1830 bp DNA linear 31-Oct-2024 DEFINITION J23119+K1357010+J23119+I13504 ACCESSION BBa_K1679021 VERSION BBa_K1679021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..736 /label=mrfp1 misc_feature 743..767 /label=Help:Barcodes CDS 62..767 /label=BBa_E1010 stem_loop 787..830 /label=stem_loop misc_feature 776..855 /label=BBa_B0010 terminator 776..855 /label=BBa_B0010 stem_loop 871..890 /label=T7 TE misc_feature 897..897 /label=stop polyA_site 891..904 /label=polya misc_feature 864..904 /label=BBa_B0012 terminator 864..904 /label=BBa_B0012 terminator 776..904 /label=BBa_B0015 misc_feature 44..904 /label=BBa_K1357010 promoter 913..947 /label=BBa_J23119 misc_feature 960..963 /label=conserved RBS 956..967 /label=BBa_B0034 CDS 974..1693 /label=GFP protein CDS 974..1693 /label=BBa_E0040 stem_loop 1713..1756 /label=stem_loop misc_feature 1702..1781 /label=BBa_B0010 terminator 1702..1781 /label=BBa_B0010 stem_loop 1797..1816 /label=T7 TE misc_feature 1823..1823 /label=stop polyA_site 1817..1830 /label=polya misc_feature 1790..1830 /label=BBa_B0012 terminator 1790..1830 /label=BBa_B0012 misc_feature 956..1830 /label=BBa_I13504 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 901 tatatactag agttgacagc tagctcagtc ctaggtataa tgctagctac tagagaaaga 961 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 1021 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 1081 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 1141 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 1201 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 1261 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 1321 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 1381 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 1441 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 1501 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 1561 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 1621 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 1681 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1801 caccttcggg tgggcctttc tgcgtttata //