LOCUS       BBa_K1679022            1440 bp    DNA     linear       27-Jan-2026
DEFINITION  KIG, kcoil+intein+gfp
ACCESSION   BBa_K1679022
VERSION     BBa_K1679022.1  
FEATURES             Location/Qualifiers
     CDS             7..720
                     /label=GFP
     CDS             721..1314
                     /label=VMA
     CDS             1321..1368
                     /label=linker
     misc_feature    1366..1428
                     /label=kcoil
ORIGIN
        1 ggatccatgc gtaaaggcga agaactgttt accggtgtgg ttccgattct ggtggaactg
       61 gacggcgatg ttaatggtca taaattcagt gttcgcggcg aaggtgaagg cgatgcgacg
      121 aacggcaaac tgaccctgaa atttatctgc accacgggta aactgccggt cccgtggccg
      181 acgctggtga ccacgctgac ctatggcgtt caatgttttg cgcgttaccc ggatcacatg
      241 aaacagcacg actttttcaa atcggccatg ccggaaggct atgtgcagga acgtacgatt
      301 agctttaaag acgatggtac gtataaaacc cgcgcggaag tgaaattcga aggcgatacc
      361 ctggttaacc gtatcgaact gaaaggtatc gatttcaaag aagacggcaa tattctgggt
      421 cataaactgg aatataactt caattcccac aacgtgtaca tcaccgcgga taaacagaaa
      481 aacggcatta aagccaattt caaaatccgc cataatgtgg aagatggtag cgttcagctg
      541 gccgaccact atcagcaaaa cacgccgatt ggtgatggcc cggtcctgct gccggacaat
      601 cactacctga gtacccagtc cgtgctgtca aaagatccga acgaaaaacg tgaccacatg
      661 gtcctgctgg aatttgtgac ggctgcgggt atcacccacg gcatggacga actgtataaa
      721 cacgctgcta aacgtcgtaa ctgcttcccg ggtgacaccc gtatcctggt tcagatcgac
      781 ggtgttccgc agaaaatcac cctgcgtgaa ctgtacgaac tgttcgaaga cgaacgttac
      841 gaaaacatgg tttacgttcg taaaaaaccg aaacgtgaaa tcaaagttta ctctatcgac
      901 ctggaaaccg gtaaagttgt tctgaccgac atcgaagacg ttatcaaagc tccggctacc
      961 gaccacctga tccgtttcga actggaagac ggtcgttctt tcgaaaccac cgttgaccac
     1021 ccggttctgg tttacgaaaa cggtcgtttc atcgaaaaac gtgctttcga agttaaagaa
     1081 ggtgacaaag ttctggtttc tgaactggaa ctggttgaac agtcttcttc ttctcaggac
     1141 aacccgaaaa acgaaaacct gggttctccg gaacacgacc agctgctgga aatcaaaaac
     1201 atcaaatacg ttcgtgctaa cgacgacttc gttttctctc tgaacgctaa aaaataccac
     1261 aacgttatca tcaacgaaaa catcgttacc cacgctgctg acggtgacga agacggcgcc
     1321 ggaggtggtg gcagtggagg tggaggttct ggaggtggtg gctctaaaat cgcagctctg
     1381 aaagagaaaa tcgcggctct gaaagaaaaa attgccgcgc tgaaggaata ataagtcgac
//