LOCUS       BBa_K1679025            1178 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7-4 +RiboJ +  GFP + LVA + 3 terminnators
ACCESSION   BBa_K1679025
VERSION     BBa_K1679025.1  
FEATURES             Location/Qualifiers
     misc_feature    18..45
                     /label=rrnB T2 terminator
     misc_feature    128..214
                     /label=rrnB T1 terminator
     misc_feature    223..270
                     /label=T7 terminator
     misc_feature    293..334
                     /label=LVA
     CDS             335..1048
                     /label=GFP
     RBS             1058..1062
                     /label=rbs
     RBS             1074..1148
                     /label=RiboJ
     promoter        1155..1172
                     /label=T7-4 promoter
ORIGIN
        1 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
       61 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
      121 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      181 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcaaaaaac ccctcaagac
      241 ccgtttagag gccccaaggg gttatgctag ttattgctca gcggtggcag cattaagcca
      301 ccagtgcgta gttttcatcg ttagcagcag gccttttata cagttcgtcc atgccgtggg
      361 tgatacccgc agccgtcaca aattccagca ggaccatgtg gtcacgtttt tcgttcggat
      421 cttttgacag cacggactgg gtactcaggt agtgattgtc cggcagcagg accgggccat
      481 caccaatcgg cgtgttttgc tgatagtggt cggccagctg aacgctacca tcttccacat
      541 tatggcggat tttgaaattg gctttaatgc cgtttttctg tttatccgcg gtgatgtaca
      601 cgttgtggga attgaagtta tattccagtt tatgacccag aatattgccg tcttctttga
      661 aatcgatacc tttcagttcg atacggttaa ccagggtatc gccttcgaat ttcacttccg
      721 cgcgggtttt atacgtacca tcgtctttaa agctaatcgt acgttcctgc acatagcctt
      781 ccggcatggc cgatttgaaa aagtcgtgct gtttcatgtg atccgggtaa cgcgcaaaac
      841 attgaacgcc ataggtcagc gtggtcacca gcgtcggcca cgggaccggc agtttacccg
      901 tggtgcagat aaatttcagg gtcagtttgc cgttcgtcgc atcgccttca ccttcgccgc
      961 gaacactgaa tttatgacca ttaacatcgc cgtccagttc caccagaatc ggaaccacac
     1021 cggtaaacag ttcttcgcct ttacgcatat gtatatctcc ttcttaaagt taattaaaca
     1081 aaattatttg tagaggctgt ttcgtcctca cggactcatc agaccggaaa gcacatccgg
     1141 tgacagctaa gcttctataa tgagtcgtat tagtcgac
//