BBa_K1679027 1 BBa_K1679027 VMA, a thermosensitive intein 2015-09-15T11:00:00Z 2015-09-17T01:15:56Z It's from Saccharomyces cerevisiae and synthesized by Genescript. It can self-splice at 18℃, means that it can catalyze its own excision from the flanking polypeptides, or exteins, as well as ligation of the exteins. We constructed this part from VMA and its exteins in original host. false false _2097_ 25072 25072 9 false Since extein sequences around the splicing junctions affect the splicing activity significantly, this VMATS part is intein with 10 residues of it's native N-exteins and C-exteins at the junction. While fusing intein to protein, users can fuse the intein with it's flanking residues (we recommand 2~3 residues). false Xihan Zhang annotation2464285 1 5' extein range2464285 1 1 30 annotation2464286 1 3' extein range2464286 1 1393 1422 annotation2464290 1 VMA TS range2464290 1 31 1392 BBa_K1679027_sequence 1 tctaactctgacgctatcatctacgttggttgcttcgctaaaggtaccaacgttctgatggctgacggttctatcgaatgcatcgaaaacatcgaagttggtaacaaagttatgggtaaagacggtcgtccgcgtgaagttatcaaactgccgcgtggtcgtgaaaccatgtactctgttgttcagaaatctcagcaccgtgctcacaaatctgactcttctcgtgaagttccggaactgctgaaattcacctgcaacgctacccacgaactggttgttcgtaccccgcgttctgttcgtcgtctgtctcgtaccatcaaaggtgttgaatacttcgaagttatcaccttcgaaatgggtcagaaaaaagctccggacggtcgtatcgttgaactggttaaagaagtttctaaatcttacccgatctctgaaggtccggaacgtgctaacgaactggttgaatcttaccgtaaagcttctaacaaagcttacttcgaatggaccatcgaagctcgtgacctgtctctgctgggttctcacgttcgtaaagctacctaccagacctacgctccgatcctgtacgaaaacgaccacttcttcgactacatgcagaaatctaaattccacctgaccatcgaaggtccgaaagttctggcttacctgcctggtctgtggatcggtgacggtctgtctgaccgtgctaccttctctgttgactctcgtgacacctctctgatggaacgtgttaccgaatacgctgaaaaactgaacctgtgcgctgaatacaaagaccgtaaagaaccgcaggttgctaaaaccgttaacctgtactctaaagttgttcgtggtaacggtatccgtaacaacctgaacaccgaaaacccgctgtgggacgctatcgttggtctgggtttcctgaaagacggtgttaaaaacatcccgtctttcctgtctaccgacaacatcggtacccgtgaaaccttcctggctggtctgatcgactctgacggttacgttaccgacgaacacggtatcaaagctaccatcaaaaccatccacacctctgttcgtgacggtctggtttctctggctcgttctctgggtctggttgtttctgttaacgctgaaccggctaaagttgacatgaacggtaccaaacacaaaatctcttacgctatctacatgtctggtggtgacgttctgctgaacgttctgtctaaatgcgctggttctaaaaaattccgtccggctccggctgctgctttcgctcgtgaatgccgtggtttctacttcgaactccaagaactgaaagaagacgactactacggtatcaccctgtctgacgactctgaccaccagttcctgctggctaaccaggttgttgttcacaactgcggtgaacgtggtaacgaaatggctgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z