BBa_K1679028 1 BBa_K1679028 T5 promoter + RBS + 6x His Tag + ftnA + λ T0 Terminator 2015-09-17T11:00:00Z 2015-09-18T07:45:11Z Synthesized by Genewiz. The design is composed of a T5 promoter regulated by double lacI repressor-binding sites, a RBS, a 6x His tag, a ftnA coding sequence and a λ T0 terminator. We created it for expression of ftnA in E.coli for a long time. false false _2097_ 25072 25072 9 true We created this device for expression of ftnA in E.coli for along time. T5 promoter is weaker than T7 promoter, so that it can exclude stress responses and adapt to long-time expression. The 6x His tag is design for Ni-chelating affinity chromatography. false Cun Wei annotation2470713 1 ftnA range2470713 1 139 621 annotation2470460 1 T5 promoter range2470460 1 1 48 annotation2470717 1 stop codons range2470717 1 627 637 annotation2470802 1 λ T0 terminator range2470802 1 643 737 annotation2470566 1 LacI operator range2470566 1 24 40 annotation2470627 1 LacI operator range2470627 1 56 72 annotation2470683 1 RBS range2470683 1 89 94 annotation2470712 1 6x His Tag range2470712 1 115 132 BBa_K1679028_sequence 1 aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacaattaaagaggagaaattaactatgagaggatcgcatcaccatcaccatcacggatccatgattgaaaaacttaatgagcagatgaacctggaactgtactcttcactgctttatcagcaaatgagcgcctggtgcagctatcataccttcgaaggtgctgccgcgttcctgcgccgtcacgcccaggaagagatgacgcatatgcagcgtctgtttgattacctgactgataccggcaatttaccgcgtattaataccgttgaatctccgtttgctgaatattcctcacttgatgaattattccaggaaacctataaacacgaacaattaatcacccagaaaattaacgaactggctcatgctgcaatgaccaatcaggactacccaacatttaatttcctgcaatggtatgtttctgagcagcatgaagaagagaaactgttcaaatcgattattgataaattaagcctggcaggcaaaagcggcgaaggtctgtattttatcgacaaagaactctctaccctcgacacacaaaactaaaagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z