LOCUS       BBa_K1679030            1733 bp    DNA     linear       22-Nov-2024
DEFINITION  J23119 + I13504 + J23119 + K115002 + E1010
ACCESSION   BBa_K1679030
VERSION     BBa_K1679030.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     misc_feature    44..918
                     /label=BBa_I13504
     promoter        927..961
                     /label=BBa_J23119
     RBS             1016..1021
                     /label=rbs
     misc_feature    992..997
                     /label=scar adaptation
     stem_loop       970..987
                     /label=stem_loop
     stem_loop       993..1021
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    970..1021
                     /label=BBa_K115002
     CDS             1028..1702
                     /label=mrfp1
     misc_feature    1709..1733
                     /label=Help:Barcodes
     CDS             1028..1733
                     /label=BBa_E1010
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagttga cagctagctc agtcctaggt ataatgctag
      961 ctactagagg gacaagcaat gcttgccttg aatagtaact tttgaatagt gattcaggag
     1021 gtactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
     1081 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1141 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1201 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1261 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1321 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1381 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1441 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1501 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1561 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1621 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1681 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgc
//