LOCUS       BBa_K1679031            1552 bp    DNA     linear       28-Nov-2024
DEFINITION  ftnA , RNA thermometer and RFP
ACCESSION   BBa_K1679031
VERSION     BBa_K1679031.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=T5 promoter
     misc_feature    24..40
                     /label=LacI operator
     misc_feature    56..72
                     /label=LacI operator
     RBS             89..94
                     /label=RBS
     misc_feature    115..132
                     /label=6x His Tag
     CDS             139..621
                     /label=ftnA
     misc_feature    627..637
                     /label=stop codons
     misc_feature    643..737
                     /label=λ T0 terminator
     misc_feature    1..737
                     /label=BBa_K1679028
     promoter        746..780
                     /label=BBa_J23119
     RBS             835..840
                     /label=rbs
     misc_feature    811..816
                     /label=scar adaptation
     stem_loop       789..806
                     /label=stem_loop
     stem_loop       812..840
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    789..840
                     /label=BBa_K115002
     CDS             847..1521
                     /label=mrfp1
     misc_feature    1528..1552
                     /label=Help:Barcodes
     CDS             847..1552
                     /label=BBa_E1010
ORIGIN
        1 aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg
       61 agcggataac aatttcacac aattaaagag gagaaattaa ctatgagagg atcgcatcac
      121 catcaccatc acggatccat gattgaaaaa cttaatgagc agatgaacct ggaactgtac
      181 tcttcactgc tttatcagca aatgagcgcc tggtgcagct atcatacctt cgaaggtgct
      241 gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg tctgtttgat
      301 tacctgactg ataccggcaa tttaccgcgt attaataccg ttgaatctcc gtttgctgaa
      361 tattcctcac ttgatgaatt attccaggaa acctataaac acgaacaatt aatcacccag
      421 aaaattaacg aactggctca tgctgcaatg accaatcagg actacccaac atttaatttc
      481 ctgcaatggt atgtttctga gcagcatgaa gaagagaaac tgttcaaatc gattattgat
      541 aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa agaactctct
      601 accctcgaca cacaaaacta aaagcttaat tagctgagct tggactcctg ttgatagatc
      661 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
      721 tttttattgg tgagaattac tagagttgac agctagctca gtcctaggta taatgctagc
      781 tactagaggg acaagcaatg cttgccttga atagtaactt ttgaatagtg attcaggagg
      841 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      901 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      961 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1021 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1081 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1141 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1201 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1261 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1321 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1381 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1441 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1501 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gc
//