BBa_K115002 1 BBa_K115002 RNA thermometer (FourU) 2008-07-14T11:00:00Z 2015-05-08T01:09:27Z Salmonella Enterica Tyhpy (CP000886.1) Released HQ 2013 Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator. false false _223_ 0 3006 9 In stock true Part of the sequence is altered because of the scar... true Bastiaan van den Berg annotation1966899 1 scar adaptation range1966899 1 23 28 annotation1966931 1 predicted stem-loop extending to one position before start codon range1966931 1 24 52 annotation1966930 1 stem_loop range1966930 1 1 18 annotation1966897 1 rbs range1966897 1 47 52 BBa_K1679031 1 BBa_K1679031 ftnA , RNA thermometer and RFP 2015-09-17T11:00:00Z 2015-09-18T07:46:36Z We assembled K1679031 which synthesized by Genewiz and other parts from Registry by 3A assembly. This device is consist of ftnA which is our magnetic receiver, RNA thermometer which is our thermo-regulator and a RFP in the role of reporter. false false _2097_ 25072 25072 9 false This device is consist of ftnA which is our magnetic receiver, RNA thermometer which is our thermo-regulator and a RFP in the role of reporter. false Jinyang Liang component2472393 1 BBa_K1679028 component2472394 1 BBa_J23119 component2472399 1 BBa_K115002 component2472402 1 BBa_E1010 annotation2472394 1 BBa_J23119 range2472394 1 746 780 annotation2472402 1 BBa_E1010 range2472402 1 847 1552 annotation2472399 1 BBa_K115002 range2472399 1 789 840 annotation2472393 1 BBa_K1679028 range2472393 1 1 737 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1679028 1 BBa_K1679028 T5 promoter + RBS + 6x His Tag + ftnA + &#955; T0 Terminator 2015-09-17T11:00:00Z 2015-09-18T07:45:11Z Synthesized by Genewiz. The design is composed of a T5 promoter regulated by double lacI repressor-binding sites, a RBS, a 6x His tag, a ftnA coding sequence and a &#955; T0 terminator. We created it for expression of ftnA in E.coli for a long time. false false _2097_ 25072 25072 9 true We created this device for expression of ftnA in E.coli for along time. T5 promoter is weaker than T7 promoter, so that it can exclude stress responses and adapt to long-time expression. The 6x His tag is design for Ni-chelating affinity chromatography. false Cun Wei annotation2470566 1 LacI operator range2470566 1 24 40 annotation2470717 1 stop codons range2470717 1 627 637 annotation2470683 1 RBS range2470683 1 89 94 annotation2470802 1 &#955; T0 terminator range2470802 1 643 737 annotation2470712 1 6x His Tag range2470712 1 115 132 annotation2470460 1 T5 promoter range2470460 1 1 48 annotation2470627 1 LacI operator range2470627 1 56 72 annotation2470713 1 ftnA range2470713 1 139 621 BBa_K1679031_sequence 1 aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacaattaaagaggagaaattaactatgagaggatcgcatcaccatcaccatcacggatccatgattgaaaaacttaatgagcagatgaacctggaactgtactcttcactgctttatcagcaaatgagcgcctggtgcagctatcataccttcgaaggtgctgccgcgttcctgcgccgtcacgcccaggaagagatgacgcatatgcagcgtctgtttgattacctgactgataccggcaatttaccgcgtattaataccgttgaatctccgtttgctgaatattcctcacttgatgaattattccaggaaacctataaacacgaacaattaatcacccagaaaattaacgaactggctcatgctgcaatgaccaatcaggactacccaacatttaatttcctgcaatggtatgtttctgagcagcatgaagaagagaaactgttcaaatcgattattgataaattaagcctggcaggcaaaagcggcgaaggtctgtattttatcgacaaagaactctctaccctcgacacacaaaactaaaagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaattactagagttgacagctagctcagtcctaggtataatgctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1679028_sequence 1 aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacaattaaagaggagaaattaactatgagaggatcgcatcaccatcaccatcacggatccatgattgaaaaacttaatgagcagatgaacctggaactgtactcttcactgctttatcagcaaatgagcgcctggtgcagctatcataccttcgaaggtgctgccgcgttcctgcgccgtcacgcccaggaagagatgacgcatatgcagcgtctgtttgattacctgactgataccggcaatttaccgcgtattaataccgttgaatctccgtttgctgaatattcctcacttgatgaattattccaggaaacctataaacacgaacaattaatcacccagaaaattaacgaactggctcatgctgcaatgaccaatcaggactacccaacatttaatttcctgcaatggtatgtttctgagcagcatgaagaagagaaactgttcaaatcgattattgataaattaagcctggcaggcaaaagcggcgaaggtctgtattttatcgacaaagaactctctaccctcgacacacaaaactaaaagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_K115002_sequence 1 ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z