BBa_K1679037 1 BBa_K1679037 VMA Dead, an inactivated intein 2015-09-16T11:00:00Z 2015-09-17T02:29:39Z We mutated a amino acid of VMA by PCR to get this part. We have submit VMA, a intein which can self-splice at 18℃, means that it can catalyze its own excision from the flanking polypeptides, or exteins, as well as ligation of the exteins. VMA Dead is an inactivated mutation of VMA, we constructed it by replace Asn-464 with Gln. We design this part as a control group of VMA.We constructed this part from VMA Dead and its exteins in original host. false false _2097_ 25072 25072 9 false VMA Dead is a inactivated mutation of VMA, we constructed it by replace Asn-464 with Gln. We design this part as a control group of VMA. We constructed this part from VMA Dead and its exteins in original host. This VMA Dead part is an inactivated intein with 10 residues of native N-exteins and C-exteins of VMA at the junction. While fusing intein to protein, users can fuse the intein with it's flanking residues (we recommand 2~3 residues). false Jinyang Liang annotation2464862 1 VMA Dead range2464862 1 31 1392 annotation2464859 1 5' extein range2464859 1 1 30 annotation2464860 1 3' extein range2464860 1 1393 1422 BBa_K1679037_sequence 1 tctaactctgacgctatcatctacgttggttgcttcgctaaaggtaccaacgttctgatggctgacggttctatcgaatgcatcgaaaacatcgaagttggtaacaaagttatgggtaaagacggtcgtccgcgtgaagttatcaaactgccgcgtggtcgtgaaaccatgtactctgttgttcagaaatctcagcaccgtgctcacaaatctgactcttctcgtgaagttccggaactgctgaaattcacctgcaacgctacccacgaactggttgttcgtaccccgcgttctgttcgtcgtctgtctcgtaccatcaaaggtgttgaatacttcgaagttatcaccttcgaaatgggtcagaaaaaagctccggacggtcgtatcgttgaactggttaaagaagtttctaaatcttacccgatctctgaaggtccggaacgtgctaacgaactggttgaatcttaccgtaaaacttctaacaaagctcacttcgaatggaccatcgaagctcgtgacctgtctctgctgggttctcacgttcgtaaagctacctaccagacctacgctccgatcctgtacgaaaacgaccacttcttcgactacatgcagaaatctaaattccacctgaccatcgaaggtccgaaagttctggcttacctgcctggtctgtggatcggtgacggtctgtctgaccgtgctaccttctctgttgactctcgtgacacctctctgatggaacgtgttaccgaatacgctgaaaaactgaacctgtgcgctgaatacaaagaccgtaaagaaccgcaggttgctaaaaccgttaacctgtactctaaagttgttcgtggtaacggtatccgtaacaacctgaacaccgaaaacccgctgtgggacgctatcgttggtctgggtttcctgaaagacggtgttaaaaacatcccgtctttcctgtctaccgacaacatcggtacccgtgaaaccttcctggctggtctgatcgactctgacggttacgttaccgacgaacacggtatcaaagctaccatcaaaaccatccacacctctgttcgtgacggtctggtttctctggctcgttctctgggtctggttgtttctgttaacgctgaaccggctaaagttgacatgaacggtaccaaacacaaaatctcttacgctatctacatgtctggtggtgacgttctgctgaacgttctgtctaaatgcgctggttctaaaaaattccgtccggctccggctgctgctttcgctcgtgaatgccgtggtttctacttcgaactccaagaactgaaagaagacgactactacggtatcaccctgtctgacgactctgaccaccagttcctgctggctaaccaggttgttgttcaccagtgcggtgaacgtggtaacgaaatggctgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z