LOCUS BBa_K1680014 4928 bp DNA linear 22-May-2024 DEFINITION pRS313 yeast shuttle vector with Biobrick MCS ACCESSION BBa_K1680014 VERSION BBa_K1680014.1 FEATURES Location/Qualifiers promoter 315..502 /label=HIS3 promotor CDS 503..1162 /label=HIS3 misc_feature 1423..1878 /label=f1 ori CDS 1661..2077 /label=lacZalpha promoter 2153..2172 /label=T3 promotor promoter 2241..2271 /label=lac promotor misc_feature 2595..3183 /label=pUC origin CDS 3354..4214 /label=ampR promoter 4215..4319 /label=ampR promotor misc_feature 4356..4859 /label=CEN/ARS yeast centromere promoter 2046..2065 /label=T7 promotor misc_feature 2083..2125 /label=RFC10 MCS ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga 241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt 301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt 361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa 421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca 481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa 541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc 601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat 661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc 721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac 781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt 841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga 901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga 961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac 1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa 1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc 1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgtagcat acgatatata 1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata 1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc 1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 aattgtaata cgactcacta tagggcgaat tggagctcac gtctgcagcg gccgctacta 2101 gtactctaga agcggccgcg aattcacgtg ggcccggtac ccagcttttg ttccctttag 2161 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 2221 tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt 2281 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 2341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 2401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 2461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 2521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 2581 gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa aaatcgacgc 2641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt cccccctgga 2701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 2761 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 2821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 2881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 2941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3001 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 3061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 3361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 3421 tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 3481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 3541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 3601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 3661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 3721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc 3781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 3841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 3901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 3961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 4021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 4081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 4141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 4201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 4261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 4321 acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa aataattata 4381 atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa 4441 gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata 4501 ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc 4561 tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa 4621 tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt 4681 gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt 4741 ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat 4801 tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc 4861 gtcctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 4921 ctttcgtc //