LOCUS BBa_K1680015 5979 bp DNA linear 23-May-2024 DEFINITION pRS315 yeast shuttle vector with Biobrick MCS ACCESSION BBa_K1680015 VERSION BBa_K1680015.1 FEATURES Location/Qualifiers CDS 663..1757 /label=LEU2 promoter 1770..2174 /label=LEU2 promotor misc_feature 2474..2929 /label=f1 origin CDS 2712..3128 /label=lacZalpha promoter 3097..3115 /label=T7 promotor misc_feature 3134..3176 /label=RFC10 CDS 3186..3248 /label=lacZalpha promoter 3205..3223 /label=T3 promotor promoter 3292..3322 /label=lac promotor misc_feature 3646..4234 /label=pUC origin CDS 4405..5265 /label=ampR promoter 5266..5370 /label=ampR promotor misc_feature 5407..5910 /label=CEN/ARS yeast centromere ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggattt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tccgattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa 3121 ttggagctca cgtctgcagc ggccgctact agtactctag aagcggccgc gaattcacgt 3181 gggcccggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc 3241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg 3301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat 3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 3481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg 3661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3721 actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc ctgttccgac 3781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3841 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4081 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4441 gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat 4501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 4741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4801 atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 4981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 5041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 5101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 5161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 5221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 5281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 5401 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa 5461 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg 5521 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc 5581 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg 5641 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt 5701 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt 5761 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca 5821 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac 5881 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat 5941 taacctataa aaataggcgt atcacgaggc cctttcgtc //