LOCUS BBa_K1680016 4848 bp DNA linear 23-May-2024 DEFINITION pRS316 yeast shuttle vector with Biobrick MCS ACCESSION BBa_K1680016 VERSION BBa_K1680016.1 FEATURES Location/Qualifiers promoter 195..410 /label=URA3 promotor CDS 411..1214 /label=URA3 misc_feature 1343..1798 /label=f1 origin CDS 1581..1997 /label=lacZalpha promoter 1966..1985 /label=T7 promotor misc_feature 2003..2045 /label=RFC10 MCS CDS 2055..2117 /label=lacZalpha promoter 2073..2092 /label=T3 promotor promoter 2161..2191 /label=lac promotor misc_feature 2515..3103 /label=pUC origin CDS 3274..4134 /label=ampR promoter 4135..4239 /label=ampR promotor misc_feature 4276..4779 /label=CEN/ARS yeast centromere ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 361 tcttaaccca actgcacaga acaaaaacct gtaggaaacg aagataaatc atgtcgaaag 421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 781 tggtggtccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg 1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca 1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 1981 tagggcgaat tggagctcac gtctgcagcg gccgctacta gtactctaga agcggccgcg 2041 aattcacgtg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt 2101 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2161 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact 2221 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2521 taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2581 cccgacagga ctataaagat accaggcgtt cccccctgga agctccctcg tgcgctctcc 2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2701 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2941 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat 3361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 3601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3661 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt 3721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 3901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 3961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 4021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4261 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa 4321 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt 4381 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt 4441 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg 4501 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc 4561 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt 4621 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat 4681 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt 4741 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta 4801 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc //