BBa_K1680021 1 BBa_K1680021 Dronpa caged Cre with NLS 2015-09-16T11:00:00Z 2016-01-26T01:27:03Z gBlock design by Team Tuebingen Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012). false false _2098_ 4206 26366 9 false 3A Assembly using RFC25 false Nicolai von Kuegelgen annotation2477375 1 linker range2477375 1 700 717 annotation2477374 1 Dronpa range2477374 1 31 699 annotation2477377 1 linker range2477377 1 1747 1764 annotation2477373 1 SV40 NLS range2477373 1 4 24 annotation2477378 1 Dronpa range2477378 1 1765 2433 annotation2477376 1 Cre recombinase range2477376 1 718 1746 BBa_K1680021_sequence 1 ccaccaaagaaaaagagaaaagttaccggctctgttatcaagccagacatgaagatcaagttgagaatggaaggtgctgttaacggtcacccattcgctatcgaaggtgttggtttgggtaagccattcgaaggtaagcaatctatggacttgaaggttaaggaaggtggtccattgccattcgcttacgacatcttgactactgttttctgttacggtaacagagttttcgctaagtacccagaaaacatcgttgactacttcaagcaatctttcccagaaggttactcttgggaaagatctatgaactacgaagacggtggtatctgtaacgctactaacgacatcactttggacggtgactgttacatctacgaaatcagattcgacggtgttaacttcccagctaacggtccagttatgcaaaagagaactgttaagtgggaaccatctactgaaaatttgtacgttagagacggtgttttgaagggtgacgttaacatggctttgtctttggaaggtggtggtcactacagatgtgacttcaagactacttacaaggctaagaaggttgttcaattgccagactaccacttcgttgaccaccacatcgaaatcaagtctcacgacaaggactactctaacgttaacttgcacgaacacgctgaagctcactctgaattgccaagacaagctaagaccggctcttctaccggcatgtctaatctgcttactgttcatcaaaatcttcctgcattacctgttgatgctacctcagacgaagttaggaaaaacttaatggatatgtttagagatagacaagctttctctgaacatacctggaaaatgttattaagcgtttgtcgttcttgggctgcatggtgtaaattaaataataggaagtggttcccagcagagcctgaagatgtgagagattatttgttgtacttacaggccagaggtttagcagtaaaaacaattcaacaacatttaggtcaattgaatatgctacacagaagatccggtttgcccagacctagcgattctaatgcagtgtctttagttatgaggagaataagaaaagaaaatgttgacgccggtgaaagagccaaacaggcattagcttttgaaaggacagatttcgatcaagtaaggtctcttatggaaaattcagataggtgccaggacatcagaaatttggcatttttaggcatagcatataatacgttgctgagaatcgcagaaatcgcaagaatcagagttaaagacatttccagaacagatggcggcagaatgttgatacatattggcagaaccaaaactttagtttccacagcaggtgtcgagaaagccttgtctttaggtgttacgaagcttgtggaaagatggatttctgtgtctggagtggcggatgacccaaataactatttattttgcagggtgaggaaaaatggggtagccgctccatctgctacttcacaattgtcaacgagagccctagagggcatcttcgaggctacacacaggttgatatatggcgccaaagatgactctggtcaaagatacttggcctggtccgggcattctgctagagtgggggcagcaagggatatggcaagggcgggtgtctctatcccagaaattatgcaagctggtggttggaccaatgtcaatatcgttatgaactatataagaaatcttgacagcgagactggagcaatggttagacttttagaagacggtgacaccggctcttctaccggctctgttatcaagccagacatgaagatcaagttgagaatggaaggtgctgttaacggtcacccattcgctatcgaaggtgttggtttgggtaagccattcgaaggtaagcaatctatggacttgaaggttaaggaaggtggtccattgccattcgcttacgacatcttgactactgttttctgttacggtaacagagttttcgctaagtacccagaaaacatcgttgactacttcaagcaatctttcccagaaggttactcttgggaaagatctatgaactacgaagacggtggtatctgtaacgctactaacgacatcactttggacggtgactgttacatctacgaaatcagattcgacggtgttaacttcccagctaacggtccagttatgcaaaagagaactgttaagtgggaaccatctactgaaaatttgtacgttagagacggtgttttgaagggtgacgttaacatggctttgtctttggaaggtggtggtcactacagatgtgacttcaagactacttacaaggctaagaaggttgttcaattgccagactaccacttcgttgaccaccacatcgaaatcaagtctcacgacaaggactactctaacgttaacttgcacgaacacgctgaagctcactctgaattgccaagacaagctaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z