BBa_K1680024 1 BBa_K1680024 Dronpa caged Cre with NLS 2015-09-16T11:00:00Z 2016-01-26T10:22:01Z gBlock design by Team Tuebingen Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012). false false _2098_ 4206 26366 9 false 3A assembly with RFC25 false Nicolai von Kuegelgen annotation2477390 1 K1680004 range2477390 1 1 24 annotation2477392 1 K1680007 range2477392 1 745 1773 annotation2477395 1 K1680003 range2477395 1 700 744 annotation2477394 1 K1680006 range2477394 1 1818 2487 annotation2477393 1 K1680006 range2477393 1 31 699 annotation2477396 1 K1680003 range2477396 1 1774 1817 BBa_K1680024_sequence 1 ccaccaaagaaaaagagaaaagttaccggctctgttatcaagccagacatgaagatcaagttgagaatggaaggtgctgttaacggtcacccattcgctatcgaaggtgttggtttgggtaagccattcgaaggtaagcaatctatggacttgaaggttaaggaaggtggtccattgccattcgcttacgacatcttgactactgttttctgttacggtaacagagttttcgctaagtacccagaaaacatcgttgactacttcaagcaatctttcccagaaggttactcttgggaaagatctatgaactacgaagacggtggtatctgtaacgctactaacgacatcactttggacggtgactgttacatctacgaaatcagattcgacggtgttaacttcccagctaacggtccagttatgcaaaagagaactgttaagtgggaaccatctactgaaaatttgtacgttagagacggtgttttgaagggtgacgttaacatggctttgtctttggaaggtggtggtcactacagatgtgacttcaagactacttacaaggctaagaaggttgttcaattgccagactaccacttcgttgaccaccacatcgaaatcaagtctcacgacaaggactactctaacgttaacttgcacgaacacgctgaagctcactctgaattgccaagacaagctaagaccggctctggttctggttctggttctggttctggttctaccggcatgtctaatctgcttactgttcatcaaaatcttcctgcattacctgttgatgctacctcagacgaagttaggaaaaacttaatggatatgtttagagatagacaagctttctctgaacatacctggaaaatgttattaagcgtttgtcgttcttgggctgcatggtgtaaattaaataataggaagtggttcccagcagagcctgaagatgtgagagattatttgttgtacttacaggccagaggtttagcagtaaaaacaattcaacaacatttaggtcaattgaatatgctacacagaagatccggtttgcccagacctagcgattctaatgcagtgtctttagttatgaggagaataagaaaagaaaatgttgacgccggtgaaagagccaaacaggcattagcttttgaaaggacagatttcgatcaagtaaggtctcttatggaaaattcagataggtgccaggacatcagaaatttggcatttttaggcatagcatataatacgttgctgagaatcgcagaaatcgcaagaatcagagttaaagacatttccagaacagatggcggcagaatgttgatacatattggcagaaccaaaactttagtttccacagcaggtgtcgagaaagccttgtctttaggtgttacgaagcttgtggaaagatggatttctgtgtctggagtggcggatgacccaaataactatttattttgcagggtgaggaaaaatggggtagccgctccatctgctacttcacaattgtcaacgagagccctagagggcatcttcgaggctacacacaggttgatatatggcgccaaagatgactctggtcaaagatacttggcctggtccgggcattctgctagagtgggggcagcaagggatatggcaagggcgggtgtctctatcccagaaattatgcaagctggtggttggaccaatgtcaatatcgttatgaactatataagaaatcttgacagcgagactggagcaatggttagacttttagaagacggtgacaccggctctggttctggttctggttctggttctggttctaccggctctgttatcaagccagacatgaagatcaagttgagaatggaaggtgctgttaacggtcacccattcgctatcgaaggtgttggtttgggtaagccattcgaaggtaagcaatctatggacttgaaggttaaggaaggtggtccattgccattcgcttacgacatcttgactactgttttctgttacggtaacagagttttcgctaagtacccagaaaacatcgttgactacttcaagcaatctttcccagaaggttactcttgggaaagatctatgaactacgaagacggtggtatctgtaacgctactaacgacatcactttggacggtgactgttacatctacgaaatcagattcgacggtgttaacttcccagctaacggtccagttatgcaaaagagaactgttaagtgggaaccatctactgaaaatttgtacgttagagacggtgttttgaagggtgacgttaacatggctttgtctttggaaggtggtggtcactacagatgtgacttcaagactacttacaaggctaagaaggttgttcaattgccagactaccacttcgttgaccaccacatcgaaatcaagtctcacgacaaggactactctaacgttaacttgcacgaacacgctgaagctcactctgaattgccaagacaagctaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z