LOCUS       BBa_K1680025            2900 bp    DNA     linear       22-Nov-2024
DEFINITION  RFP-luciferase Cre reporter
ACCESSION   BBa_K1680025
VERSION     BBa_K1680025.1  
FEATURES             Location/Qualifiers
     promoter        1..1445
                     /label=Constitutive promoter
     misc_feature    1348..1354
                     /label=TATA Box
     promoter        1..1445
                     /label=BBa_J63005
     misc_feature    1454..1487
                     /label=BBa_K1680005
     CDS             1494..2168
                     /label=mrfp1
     misc_feature    2175..2199
                     /label=Help:Barcodes
     CDS             1494..2199
                     /label=BBa_E1010
     terminator      2208..2336
                     /label=BBa_K392003
     misc_feature    2345..2378
                     /label=BBa_K1680005
     CDS             2385..2900
                     /label=NanoLuc
     CDS             2385..2900
                     /label=BBa_K1680009
ORIGIN
        1 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
       61 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
      121 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
      181 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
      241 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
      301 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
      361 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
      421 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
      481 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
      541 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
      601 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
      661 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
      721 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
      781 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
      841 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
      901 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
      961 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     1021 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     1081 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg
     1141 aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt
     1201 atcttcgaac acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc
     1261 aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt
     1321 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     1381 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     1441 caacttacta gagataactt cgtatagcat acattatacg aagttattac tagatggctt
     1501 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
     1561 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     1621 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
     1681 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
     1741 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
     1801 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
     1861 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
     1921 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
     1981 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
     2041 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
     2101 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     2161 ccggtgctta ataacgctga tagtgctagt gtagatcgct actagaggca tgtgctctgt
     2221 atgtatataa aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattagga
     2281 cctttgcagc ataaattact atacttctat tactagagcc cgccgccacc atggagtact
     2341 agagataact tcgtatagca tacattatac gaagttatta ctagatggtc ttcaccttag
     2401 aagactttgt tggtgactgg aggcaaactg ccggttataa tcttgaccaa gttttggaac
     2461 aaggtggtgt atcatcactt ttccagaatc taggagttag cgtcacaccc attcaaagaa
     2521 tcgtcctatc tggcgaaaat ggtttaaaaa ttgatattca tgttattatt ccctatgaag
     2581 gtttatcagg agatcaaatg ggtcagatcg agaagatttt taaggtggta tatcctgtcg
     2641 atgaccacca cttcaaagtc attttgcatt atggtacttt ggttattgac ggcgtaaccc
     2701 ctaacatgat tgattacttc ggtagacctt atgaggggat agcagttttt gatggtaaaa
     2761 aaattacagt cacagggact ttatggaacg gcaataaaat catcgacgaa cgtctaatca
     2821 atccagatgg atcattgtta ttcagggtta ccattaatgg agttactggt tggagattgt
     2881 gtgagcgtat tttggcttaa
//