BBa_K1680005 1 BBa_K1680005 loxP Site 2015-09-16T11:00:00Z 2015-10-28T06:30:39Z gBlock designed by Team Tuebingen This part contains the WT loxP site, which is recognised by Cre recombinase (Part BBa_K1680007), in forward orientation. false false _2098_ 26366 26460 9 false Part is shipped in RFC10. false Katharina Sporbeck BBa_K1680009 1 BBa_K1680009 NanoLuc Luciferase 2015-09-16T11:00:00Z 2015-10-27T01:37:07Z gBlock designed by Team Tuebingen, sequence by Promega Protein coding region for the NanoLuc(tm) Luziferase (designed by the Promega Corporation, free for research use), Codon optimized for yeast. The Nanoluc is smaller than other luziferase proteins and yields equal or stronger luminescence readings. false false _2098_ 15693 26460 9 false Part is shipped in RFC10. false Katharina Sporbeck annotation2477413 1 NanoLuc range2477413 1 1 516 BBa_J63005 1 P0310 yeast ADH1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic DNA of ADH1 from S. cerevisiae ADH1 promoter from S. cerevisiae false true _97_ 0 545 97 It's complicated false no changes to genomic sequence true Caroline Ajo-Franklin annotation1902745 1 TATA Box range1902745 1 1348 1354 annotation1902744 1 Constitutive promoter range1902744 1 1 1445 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K1680025 1 BBa_K1680025 RFP-luciferase Cre reporter 2015-09-16T11:00:00Z 2015-10-28T03:54:52Z designed by Team Tuebingen This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase. false false _2098_ 15693 26366 9 false RFC10 assembly false Nicolai von Kuegelgen component2466051 1 BBa_K392003 component2466050 1 BBa_E1010 component2466046 1 BBa_J63005 component2466053 1 BBa_K1680009 component2466047 1 BBa_K1680005 component2466052 1 BBa_K1680005 annotation2466047 1 BBa_K1680005 range2466047 1 1454 1487 annotation2466046 1 BBa_J63005 range2466046 1 1 1445 annotation2466053 1 BBa_K1680009 range2466053 1 2385 2900 annotation2466051 1 BBa_K392003 range2466051 1 2208 2336 annotation2466050 1 BBa_E1010 range2466050 1 1494 2199 annotation2466052 1 BBa_K1680005 range2466052 1 2345 2378 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K1680005_sequence 1 ataacttcgtatagcatacattatacgaagttat BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1680025_sequence 1 aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaacttactagagataacttcgtatagcatacattatacgaagttattactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggagtactagagataacttcgtatagcatacattatacgaagttattactagatggtcttcaccttagaagactttgttggtgactggaggcaaactgccggttataatcttgaccaagttttggaacaaggtggtgtatcatcacttttccagaatctaggagttagcgtcacacccattcaaagaatcgtcctatctggcgaaaatggtttaaaaattgatattcatgttattattccctatgaaggtttatcaggagatcaaatgggtcagatcgagaagatttttaaggtggtatatcctgtcgatgaccaccacttcaaagtcattttgcattatggtactttggttattgacggcgtaacccctaacatgattgattacttcggtagaccttatgaggggatagcagtttttgatggtaaaaaaattacagtcacagggactttatggaacggcaataaaatcatcgacgaacgtctaatcaatccagatggatcattgttattcagggttaccattaatggagttactggttggagattgtgtgagcgtattttggcttaa BBa_J63005_sequence 1 aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact BBa_K1680009_sequence 1 atggtcttcaccttagaagactttgttggtgactggaggcaaactgccggttataatcttgaccaagttttggaacaaggtggtgtatcatcacttttccagaatctaggagttagcgtcacacccattcaaagaatcgtcctatctggcgaaaatggtttaaaaattgatattcatgttattattccctatgaaggtttatcaggagatcaaatgggtcagatcgagaagatttttaaggtggtatatcctgtcgatgaccaccacttcaaagtcattttgcattatggtactttggttattgacggcgtaacccctaacatgattgattacttcggtagaccttatgaggggatagcagtttttgatggtaaaaaaattacagtcacagggactttatggaacggcaataaaatcatcgacgaacgtctaatcaatccagatggatcattgttattcagggttaccattaatggagttactggttggagattgtgtgagcgtattttggcttaa BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z