LOCUS       BBa_K1680026            3324 bp    DNA     linear       22-Nov-2024
DEFINITION  pETUE
ACCESSION   BBa_K1680026
VERSION     BBa_K1680026.1  
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label=T7 promoter
     misc_feature    20..44
                     /label=Lac operator
     RBS             53..67
                     /label=RBS.1 strong
     misc_feature    77..94
                     /label=6x His-tag
     misc_feature    104..121
                     /label=thrombin cleavage site
     promoter        212..240
                     /label=tet promoter
     misc_feature    410..496
                     /label=rrnB T1 terminator
     misc_feature    683..730
                     /label=T7 terminator
     misc_feature    767..1222
                     /label=f1 ori
     misc_feature    1337..2197
                     /label=AmpR
     misc_feature    2290..2878
                     /label=ori
     misc_feature    3277..3296
                     /label=lac operator
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta ggattaaaga
       61 ggagaaatct aggatgcatc atcatcatca tcacagcagc ggcctggtgc cgcgcggcag
      121 ccccgggtaa tactgcaggc cgggcgttgt aatcatagtc ataatcaata ctcctgactg
      181 cgttagcaat ttaactgtga taaactaccg cattaaagct attcgatgat aagctgtcaa
      241 acatgataat tcttgaagac gaaagggcct aggctgataa aacagaattt gcctggcggc
      301 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
      361 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
      421 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      481 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      541 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      601 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
      661 atatgtatcc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      721 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      781 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      841 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      901 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      961 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1021 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1081 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1141 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1201 aaaatattaa cgtttacaat ttctggcggc acgatggcat gagattatca aaaaggatct
     1261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1621 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1921 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     1981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatcatgac
     2221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2401 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagccggcga
     3061 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     3121 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     3181 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     3241 taaagaagac agtcataagt gcggcgacga ccggtgaatt gtgagcgctc acaattctcg
     3301 tgacatcata acgtcccgcg aaat
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