##gff-version 3
##sequence-region BBa_K1680026 1 3296
BBa_K1680026	.	promoter	1	19	.	+	0	ID=annotation2472193;Name=T7 promoter
BBa_K1680026	.	sequence_feature	104	121	.	+	0	ID=annotation2472237;Name=thrombin cleavage site
BBa_K1680026	.	sequence_feature	2290	2878	.	+	0	ID=annotation2472305;Name=ori
BBa_K1680026	.	tag	77	94	.	+	0	ID=annotation2472216;Name=6x His-tag
BBa_K1680026	.	sequence_feature	1337	2197	.	+	0	ID=annotation2472289;Name=AmpR
BBa_K1680026	.	sequence_feature	767	1222	.	+	0	ID=annotation2472261;Name=f1 ori
BBa_K1680026	.	operator	20	44	.	+	0	ID=annotation2472194;Name=Lac operator
BBa_K1680026	.	stop_codon	683	730	.	+	0	ID=annotation2472260;Name=T7 terminator
BBa_K1680026	.	promoter	212	240	.	+	0	ID=annotation2472238;Name=tet promoter
BBa_K1680026	.	stop_codon	410	496	.	+	0	ID=annotation2472239;Name=rrnB T1 terminator
BBa_K1680026	.	operator	3277	3296	.	+	0	ID=annotation2472328;Name=lac operator
BBa_K1680026	.	ribosome_entry_site	53	67	.	+	0	ID=annotation2472195;Name=RBS.1 strong
>BBa_K1680026
taatacgactcactataggggaattgtgagcggataacaattcccctctaggattaaagaggagaaatctaggatgcat
catcatcatcatcacagcagcggcctggtgccgcgcggcagccccgggtaatactgcaggccgggcgttgtaatcatag
tcataatcaatactcctgactgcgttagcaatttaactgtgataaactaccgcattaaagctattcgatgataagctgt
caaacatgataattcttgaagacgaaagggcctaggctgataaaacagaatttgcctggcggcagtagcgcggtggtcc
cacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagg
gaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcagga
cgcccgccataaactgccaggcatcaaattaagcagaaggccatcctgacggatggcctttttgcgtttctacaaactc
ttttgtttatttttctaaatacattcaaatatgtatccgctgagcaataactagcataaccccttggggcctctaaacg
ggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagcggcgcattaa
gcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttctt
cccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagt
gctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggttt
ttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctc
ggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaattt
aacgcgaattttaacaaaatattaacgtttacaatttctggcggcacgatggcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaa
tgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtaga
taactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccaga
tttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggca
tcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccc
catgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc
atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaa
ccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgaga
tccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaa
aaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttca
atcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttctt
gagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgga
tcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtag
ccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctg
ctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctg
aacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatga
gaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacga
gggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattttt
gtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctgg
ccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgata
ccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagccggcgataatggcctgcttctcgccga
aacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgat
catcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatg
ataaagaagacagtcataagtgcggcgacgaccggtgaattgtgagcgctcacaattctcgtgacatcataacgtcccg
cgaaat