LOCUS       BBa_K1682008             962 bp    DNA     linear       26-Dec-2024
DEFINITION  <i>P<sub>kdpF</sub>[-15, T>C]</i> - GFP generator
ACCESSION   BBa_K1682008
VERSION     BBa_K1682008.1  
FEATURES             Location/Qualifiers
     promoter        69..74
                     /label=-10
     promoter        42..47
                     /label=-35
     misc_feature    8..24
                     /label=phospho-KdpE binding site
     misc_feature    65..66
                     /label=EcoRI Illegal site removed T->C
     promoter        1..78
                     /label=BBa_K1682003
     RBS             87..99
                     /label=RBS-3\Weak
     RBS             93..96
                     /label=RBS
     misc_feature    87..99
                     /label=BBa_B0032
     RBS             87..99
                     /label=BBa_B0032
     CDS             106..825
                     /label=GFP protein
     CDS             106..825
                     /label=BBa_E0040
     stem_loop       845..888
                     /label=stem_loop
     misc_feature    834..913
                     /label=BBa_B0010
     terminator      834..913
                     /label=BBa_B0010
     stem_loop       929..948
                     /label=T7 TE
     misc_feature    955..955
                     /label=stop
     polyA_site      949..962
                     /label=polya
     misc_feature    922..962
                     /label=BBa_B0012
     terminator      922..962
                     /label=BBa_B0012
     misc_feature    87..962
                     /label=BBa_E0240
ORIGIN
        1 tgccattttt atactttttt tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca
       61 gaactctacc cttccggtta ctagagtcac acaggaaagt actagatgcg taaaggagaa
      121 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      181 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      241 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      301 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      361 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      421 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      481 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      541 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      601 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      661 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      721 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      781 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca
      841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      961 ta
//