BBa_K1682010 1 BBa_K1682010 conjugation(RP4) plasmid with MHT (Methyl halide transferase) and GFP 2015-08-25T11:00:00Z 2015-09-06T05:02:57Z MHT sequence comes from organism Batis maritima. GFP comes from the biobrick BBa_E0040. RP4 comes from gBlock pRZ101 This plasmid contains three main components: the RP4, GFP, and MHT. The RP4 gene, when transformed specifically into S17 E. coli strain, facilitates the conjugation of E.coli with most other bacteria. The plasmid also contains MHT, short for methyl halide transferase, which if provided with either Br, Cl, or I, produces its corresponding methyl halide gas. This part also contains a GFP, specifically BBa_E0040. This plasmid can be used to faciliate transformation of bacteria that are not easily transformable through heat shock or electroporation. In addition, the plasmid also have two reporter genes, which produce the GFP and the methyl halide gas. true false _2100_ 25195 25195 9 false N/A false Zilu Peter Tang annotation2438311 1 Methyl Halide Transferase range2438311 1 1783 2535 annotation2438310 1 GFP BBa_0040 range2438310 1 711 1430 annotation2438309 1 RP4 gene range2438309 1 1 688 annotation2438308 1 ATG start codon range2438308 1 1 3 BBa_K1682010_sequence 1 atgagcaccgtggcgaacattgcgccggtgtttaccggcgattgcaaaaccattccgaccccggaagaatgcgcgacctttctgtataaagtggtgaacagcggcggctgggaaaaatgctgggtggaagaagtgattccgtgggatctgggcgtgccgaccccgctggtgctgcatctggtgaaaaacaacgcgctgccgaacggcaaaggcctggtgccgggctgcggcggcggctatgatgtggtggcgatggcgaacccggaacgctttatggtgggcctggatattagcgaaaacgcgctgaaaaaagcgcgcgaaacctttagcaccatgccgaacagcagctgctttagctttgtgaaagaagatgtgtttacctggcgcccggaacagccgtttgattttatttttgattatgtgtttttttgcgcgattgatccgaaaatgcgcccggcgtggggcaaagcgatgtatgaactgctgaaaccggatggcgaactgattaccctgatgtatccgattaccaaccatgaaggcggcccgccgtttagcgtgagcgaaagcgaatatgaaaaagtgctggtgccgctgggctttaaacagctgagcctggaagattatagcgatctggcggtggaaccgcgcaaaggcaaagaaaaactggcgcgctggaaaaaaatgaacaactaataaattaaagaggagaaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttccgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagggaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatgtacaagtaagcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcccctacgtgctgctgaagttgcccgcaacagagagtggaaccaaccggtgataccacgatactatgactgagagtcaacgccatgagcggcctcatttcttattctgagttacaacagtccgcaccgctgtctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagcggccgcatgtacttcaccagctccgcgaagtcgctcttcttgatggagcgcatggggacgtgcttggcaatcacgcgcaccccccggccgttttagcggctaaaaaagtcatggctctgccctcgggcggaccacgcccatcatgaccttgccaagctcgtcctgcttctcttcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggccgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z