BBa_K1682014 1 BBa_K1682014 Conjugation(RP4) plasmid with partial GFP 2015-09-07T11:00:00Z 2015-09-18T02:12:38Z RP4 is orignially from pRZ101, and it includes both BBa_J01001 and BBa_K125320. GFP is from biobrick BBa_E0040. The RBS is from BB0030 and the terminator is from BB0014 This plasmid contains three main components: the RP4 and GFP. The RP4 gene, originally from pRZ101, when transformed specifically into S17 E. coli strain, facilitates the conjugation of E.coli with most other bacteria. It consists of TraJR BBa_J01001 and oriT BBa_K125320. The GFP is from biobrick BBa_E0040. The plasmid also contains biobrick part BB0030 RBS, and BBa_0014 Terminator. false false _2100_ 26215 25195 9 false The designed plasmid allow conjugation between the E. coli with most other bacteria. false Zilu Peter Tang annotation2447469 1 BBa_K125320 range2447469 1 1198 1296 annotation2447468 1 RP4 pRZ 101 range2447468 1 646 1395 annotation2447470 1 TraJr BBa_J01001 range2447470 1 748 1119 annotation2447466 1 part of GFP BBa_E0040 range2447466 1 16 289 annotation2447345 1 B0030 range2447345 1 1 15 annotation2447467 1 B0014 Terminator range2447467 1 599 633 BBa_K1682014_sequence 1 attaaagaggagaaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttccgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtttgtacaagtaagcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcccctacgtgctgctgaagttgcccgcaacagagagtggaaccaaccggtgataccacgatactatgactgagagtcaacgccatgagcggcctcatttcttattctgagttacaacagtccgcaccgctgtctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagcggccgcatgtacttcaccagctccgcgaagtcgctcttcttgatggagcgcatggggacgtgcttggcaatcacgcgcaccccccggccgttttagcggctaaaaaagtcatggctctgccctcgggcggaccacgcccatcatgaccttgccaagctcgtcctgcttctcttcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggccgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z