LOCUS BBa_K1682017 4529 bp DNA linear 07-May-2024
DEFINITION ParaBAD-GFP-Plac-RFP
ACCESSION BBa_K1682017
VERSION BBa_K1682017.1
FEATURES Location/Qualifiers
promoter 1080..1210
/label=Pbad
promoter 1043..1114
/label=PC
misc_feature 1..1079
/label=AraC
promoter 1..1210
/label=BBa_I0500
misc_feature 1223..1226
/label=conserved
RBS 1219..1230
/label=BBa_B0034
CDS 1237..1956
/label=GFP protein
CDS 1237..1956
/label=BBa_E0040
stem_loop 1976..2019
/label=stem_loop
misc_feature 1965..2044
/label=BBa_B0010
terminator 1965..2044
/label=BBa_B0010
stem_loop 2060..2079
/label=T7 TE
misc_feature 2086..2086
/label=stop
polyA_site 2080..2093
/label=polya
misc_feature 2053..2093
/label=BBa_B0012
terminator 2053..2093
/label=BBa_B0012
misc_feature 1..2093
/label=BBa_I13540
CDS 2102..2189
/label=end of LacI coding region (inactive)
misc_feature 2102..2301
/label=BBa_R0010
misc_feature 2190..2227
/label=CAP binding site
promoter 2238..2243
/label=-35
promoter 2262..2267
/label=-10
misc_feature 2267..2301
/label=LacI binding site
misc_feature 2274..2274
/label=start
promoter 2102..2301
/label=BBa_R0010
misc_feature 2314..2317
/label=conserved
RBS 2310..2321
/label=BBa_B0034
CDS 2328..3002
/label=mrfp1
misc_feature 3009..3033
/label=Help:Barcodes
CDS 2328..3008
/label=BBa_E1010
stem_loop 3053..3096
/label=stem_loop
misc_feature 3042..3121
/label=BBa_B0010
terminator 3042..3121
/label=BBa_B0010
stem_loop 3137..3156
/label=T7 TE
misc_feature 3163..3163
/label=stop
polyA_site 3157..3170
/label=polya
misc_feature 3130..3170
/label=BBa_B0012
terminator 3130..3170
/label=BBa_B0012
misc_feature 2102..3170
/label=BBa_J04450
promoter 3179..3213
/label=BBa_J23101
misc_feature 3226..3229
/label=conserved
RBS 3222..3233
/label=BBa_B0034
misc_feature 4329..4367
/label=LVA
CDS 3240..4367
/label=lacI-LVA
misc_feature 3240..4367
/label=BBa_C0012
misc_feature 4368..4392
/label=Help:Barcodes
CDS 3240..4367
/label=BBa_C0012
stem_loop 4412..4455
/label=stem_loop
misc_feature 4401..4480
/label=BBa_B0010
terminator 4401..4480
/label=BBa_B0010
stem_loop 4496..4515
/label=T7 TE
misc_feature 4522..4522
/label=stop
polyA_site 4516..4529
/label=polya
misc_feature 4489..4529
/label=BBa_B0012
terminator 4489..4529
/label=BBa_B0012
misc_feature 3222..4529
/label=BBa_P0412
misc_feature 2102..4529
/label=BBa_K1682016
ORIGIN
1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
1921 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
1981 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
2041 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
2101 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2161 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
2221 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
2281 agcggataac aatttcacac atactagaga aagaggagaa atactagatg gcttcctccg
2341 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
2401 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
2461 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
2521 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
2581 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
2641 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
2701 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
2761 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
2821 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
2881 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
2941 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
3001 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
3061 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
3121 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
3181 tacagctagc tcagtcctag gtattatgct agctactaga gaaagaggag aaatactaga
3241 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
3301 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
3361 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
3421 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
3481 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
3541 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
3601 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
3661 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
3721 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
3781 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
3841 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
3901 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
3961 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
4021 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
4081 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
4141 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
4201 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
4261 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4321 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct
4381 agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
4441 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
4501 accttcgggt gggcctttct gcgtttata
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