##gff-version 3
##sequence-region BBa_K1682017 1 4529
BBa_K1682017	.	engineered_region	1	2093	.	+	0	ID=BBa_I13540;Name=BBa_I13540
BBa_K1682017	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13540
BBa_K1682017	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1682017	.	terminator	2053	2093	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13540
BBa_K1682017	.	engineered_region	2053	2093	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1682017	.	stem_loop	2060	2079	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1682017	.	polyA_site	2080	2093	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1682017	.	stop_codon	2086	2086	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1682017	.	CDS	1237	1956	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13540
BBa_K1682017	.	CDS	1237	1956	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1682017	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I13540
BBa_K1682017	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K1682017	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K1682017	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K1682017	.	terminator	1965	2044	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13540
BBa_K1682017	.	engineered_region	1965	2044	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1682017	.	stem_loop	1976	2019	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1682017	.	engineered_region	2102	4529	.	+	0	ID=BBa_K1682016;Name=BBa_K1682016
BBa_K1682017	.	engineered_region	3222	4529	.	+	0	ID=BBa_P0412;Name=BBa_P0412;Parent=BBa_K1682016
BBa_K1682017	.	terminator	4489	4529	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K1682017	.	engineered_region	4489	4529	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1682017	.	stem_loop	4496	4515	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1682017	.	polyA_site	4516	4529	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1682017	.	stop_codon	4522	4522	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1682017	.	terminator	4401	4480	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K1682017	.	engineered_region	4401	4480	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1682017	.	stem_loop	4412	4455	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1682017	.	ribosome_entry_site	3222	3233	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K1682017	.	conserved_region	3226	3229	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1682017	.	CDS	3240	4367	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K1682017	.	CDS	3240	4367	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1682017	.	engineered_region	3240	4367	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1682017	.	engineered_tag	4368	4392	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1682017	.	tag	4329	4367	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1682017	.	engineered_region	2102	3170	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1682016
BBa_K1682017	.	terminator	3130	3170	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1682017	.	engineered_region	3130	3170	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1682017	.	stem_loop	3137	3156	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1682017	.	polyA_site	3157	3170	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1682017	.	stop_codon	3163	3163	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1682017	.	CDS	2328	3008	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1682017	.	engineered_tag	3009	3033	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1682017	.	CDS	2328	3002	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1682017	.	terminator	3042	3121	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1682017	.	engineered_region	3042	3121	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1682017	.	stem_loop	3053	3096	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1682017	.	ribosome_entry_site	2310	2321	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1682017	.	conserved_region	2314	2317	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1682017	.	promoter	2102	2301	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1682017	.	engineered_region	2102	2301	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1682017	.	CDS	2102	2189	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1682017	.	non_covalent_binding_site	2267	2301	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1682017	.	non_covalent_binding_site	2190	2227	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1682017	.	promoter	2262	2267	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1682017	.	promoter	2238	2243	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1682017	.	start_codon	2274	2274	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1682017	.	promoter	3179	3213	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1682016
>BBa_K1682017
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtt
agctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataaca
atttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttca
aagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtac
ccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtac
ggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggg
aacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatcta
caaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcc
accgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcact
acgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaact
ggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct
taataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactag
atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtgg
tgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaa
ccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg
tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcg
gcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgga
tgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacaccc
atcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaa
tcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaa
tcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaat
gagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccg
ggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaac
caccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtg
aagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcccc
gcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaa
ctacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata