LOCUS       BBa_K1682018            2831 bp    DNA     linear       22-Dec-2024
DEFINITION  PLTet0-1:NarL, PdcuS:sfGFP, J23109:TetR
ACCESSION   BBa_K1682018
VERSION     BBa_K1682018.1  
FEATURES             Location/Qualifiers
     promoter        37..74
                     /label=PLTet0-1
     RBS             111..144
                     /label=sRBS TIR900
     misc_feature    145..795
                     /label=NarL CDS
ORIGIN
        1 aggcgtatca cgaggccctt tcgtcttcac ctcgagtccc tatcagtgat agagattgac
       61 atccctatca gtgatagaga tactgagcac atcagcagga cgcactgacc taaggatttt
      121 tactagaaga attatataca acaaatgagt aatcaggaac cggctactat cctgctgatt
      181 gacgatcacc cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc
      241 accgtggttg gcgaagcgag taatggcgaa cagggtattg aactggcgga gtctcttgat
      301 cccgatctga tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat
      361 aaactgcgcg aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa
      421 gaagatgtgg tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa
      481 ccggaagatc tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa
      541 gcattaacgc ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat
      601 gttaaccagt taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg
      661 aacaagatga ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag
      721 cacatgctga agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag
      781 gagcgcattt tctgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tatagtaaat cactgcataa ttcgtgtcgc tcaaggcgca
      961 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga
     1021 aatgagctgg ttagctagtc aagagtctct tcccctatat agccaatcat tcttactccc
     1081 tttgaattac ccgcctcatc agagataatg cttaagaaaa agaggagaaa tactagatgc
     1141 gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg
     1201 tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac
     1261 tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg actctggtaa
     1321 cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg
     1381 acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg
     1441 atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc
     1501 gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc cataagctgg
     1561 aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta
     1621 aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact
     1681 accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga
     1741 gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg
     1801 agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa tgatgaccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1981 ttataggtac ccgtggatcc actagagtcg accagcaggc atgcaagctt tttacggcta
     2041 gctcagtcct aggtatagtg ctagcccagc cagagaaaca ctctttaaca gggggtagta
     2101 tgatgtctcg tttagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     2161 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
     2221 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     2281 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     2341 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     2401 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     2461 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgca gtggggcatt
     2521 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     2581 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     2641 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     2701 aacttaaatg tgaaagtggg tcttaaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2761 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2821 gccgccctag a
//