BBa_K1683001 1 BBa_K1683001 E. coli tolC signaling sequence + Yersenia Pestis tolC 2015-09-16T11:00:00Z 2015-09-16T11:36:46Z BBa_K1406000 comes from the biobrick registry The tolC signaling sequence and Yersenia pestis tolC sequence were synthesized from DNA sequences found in genomic databases. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Yersenia pestis Angola. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false The main consideration when designing the signaling sequence and Yersenia pestis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen BBa_K1683001_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaacctgctccaagtatacaaacaggcgcgcgactcgaatccagacctccgtaaagccgccgcagaccgtgacgctgcctatgaaaaaatcaatgaagtgcgtagcccactgctgccgcagttaggtctctcggcgggctatacacatgcgaacggctttcgtgacgcaagtaacagcccggattcaaacgcgacctccggatctctgaaactcacccagactatttttgacatgagcaaatggcgtgctctgacactacaggaaaaagcggcgggtattcaagacgtgactttccagacgtcagaacaacagctgattcttaacaccgctacggcttactttaacgtcttgcgtgcaatcgatagtttgtcttatactgaagcacagaaacagagtgtataccgtcagctggctcagaccacacaacgctttaatgtgggtctggtagcaatcactgatgtccagaatgcccgcgcatcctacgacaccgttctggcggcagaagtagccgcgcgcaacaacctcgataacgccctggaatctttacgccaaattactggcgtgtattatccggagttggcaagtctgaacgtagaacgcttaaaaacgcagcgtccagatgcggtcaacaatttgctgaaagaagcggagaaacgtaacctgagtttgctgtccgcacgtctcagccaggatttagcacgcgagcagatcaaatccgcagaaaccggttatatgccgaccgttgacttgaccgcgtcctcgtccatcacgaacacccgttattccggaggcaccccgtccagtcagcaagtgaataatgattcaggccagaaccagattggcgtacagttctccttgccattgtatagcggcggagctactaatagcgcggttaaacaggcccaatataacttcgtgggcgccagcgagcttctggaaagcgctcatcgcaatatggtacagaccttgcgcagtagttttaataacatttcagcctctatctcaagcattaacgcgtaccagcaagtcgtcatcagtaaccaatcgagccttgatgccatggaagcaggttaccaggttggtactcgtacgattttggatgtgctgaccgcaaccactaatttataccagagtaaacagcagctcgcggatgcacgctacaattatctgatcaaccaactgaatatcaagagcgcgttaggaaccctcaacatgaatgacctgatggcgcttaatgcggtgcttgataagccggtaccgacgtctgctgctgcccttgcaccagaaaacactacacgccagacggtgaccaccccccgcgcgcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z