BBa_K1683002 1 BBa_K1683002 pBAD+Strong RBS+E. coli tolC signal sequence+P. mirabilis tolC 2015-09-16T11:00:00Z 2015-09-16T11:51:46Z BBa_K1406000 comes from the biobrick registry The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases. Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Proteus mirabilis tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Proteus mirabilis. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen component2464026 1 BBa_K1683003 component2464025 1 BBa_K1406000 annotation2464025 1 BBa_K1406000 range2464025 1 1 151 annotation2464026 1 BBa_K1683003 range2464026 1 152 1549 BBa_K1406000 1 BBa_K1406000 pBAD promoter + strong RBS 2014-10-08T11:00:00Z 2015-05-08T01:10:17Z E. coli A combinatorial part of K206000, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pBAD promoter and the strong RBS using the restriction enzymes NheI and PstI. false false _1784_ 0 17066 9 It's complicated false We say we are hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick. false Sierra Tackett annotation2408503 1 promoter range2408503 1 1 131 annotation2408505 1 AraI2 range2408505 1 61 78 annotation2408508 1 BBa_B0034 range2408508 1 139 150 annotation2408506 1 BBa_K206000 range2408506 1 1 130 annotation2408507 1 conserved range2408507 1 143 146 annotation2408504 1 AraI1 range2408504 1 40 57 BBa_K1683003 1 BBa_K1683003 E. coli tolC signaling sequence + Proteus mirabilis tolC 2015-09-16T11:00:00Z 2015-09-16T11:48:31Z The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Proteus mirabilis. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen BBa_K1683002_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagcatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgaagaccttttgcaagtctaccagaaagcaaaagacagcaacccggagttgcgtaaatcacttgcggaacgcaatcaggcgttcgaaaaaattaatgaagcccgctctccgctgctgcctcagctcggcctgggagcctcaattgattataaaagtggttaccgtgatgccaaaaataccgagtctaattcgctgggagcgaacctgacactgacccaaaccatctttgacatgagcctgtggcgccagcttaccatgcaagagaaaacagcgggcatgagcgatgttacctatcaaacatctcaacagcaattaattctggataccgcgactgcgtactttaatgtgctgcgtgcgattgatagcctgagtttcattgaggcgcagaaagaacaggtctatcgccagctggaccaaacgacgcagcgctttaatgttggtctggtcgcaattactgatgtgcaaaacgctcgtgcgaattatgacagcgtgctggcacaagaagtggcaggtcgtaatgagctggacaacgcactggaaaaactccgccaggtgtcgggggtttattacattaatctggcctctctgaatattgcccgtttttcgacgaccccgcctgacgcgatcgataaattactcaaagatgctgaagaacgtaatttgagccttctgtccgcgcgcctgggccaagacctcgcgcgtgaaaacattcgccttgcgcaatcaggccatctgccaaccgtcaatctgaatgcgtctaccggcgttagtaacagccacaatcatgggtctgcgttaccgccggaaacgccggccaactcacgtaacagttacaatggccaaagctccatcggtttatcgctgagcattcctctgtacacgggtggtcgtaccagctcgcaggtcgagcaagctcagtacggttttaccagtgcatcggaacagcttgaatcggtgtatcgcagcattgttcagattgcgcgcagtagctataataatattagtgcgagcatctcctcgatcaaagcatatcaacaagtcgtcgtgtccgcgcaaagtagcttagacgcaacagaagcgggctaccaggtcgggacccgcactatcgtcgatgtattaaacgcgaccaccaccctgtacgatgcaaaacagaaactgtcgtcagcacgttatgattaccttattaatcaacttaacattcaatacgctcgcggcacgctgaacgaaaatgacctgatccagctgaataacgctctgggtgaggagatctcgaccagcccagataacattatccgtccccttacgagcccggttctgaatactaccaattaa BBa_K1683003_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgaagaccttttgcaagtctaccagaaagcaaaagacagcaacccggagttgcgtaaatcacttgcggaacgcaatcaggcgttcgaaaaaattaatgaagcccgctctccgctgctgcctcagctcggcctgggagcctcaattgattataaaagtggttaccgtgatgccaaaaataccgagtctaattcgctgggagcgaacctgacactgacccaaaccatctttgacatgagcctgtggcgccagcttaccatgcaagagaaaacagcgggcatgagcgatgttacctatcaaacatctcaacagcaattaattctggataccgcgactgcgtactttaatgtgctgcgtgcgattgatagcctgagtttcattgaggcgcagaaagaacaggtctatcgccagctggaccaaacgacgcagcgctttaatgttggtctggtcgcaattactgatgtgcaaaacgctcgtgcgaattatgacagcgtgctggcacaagaagtggcaggtcgtaatgagctggacaacgcactggaaaaactccgccaggtgtcgggggtttattacattaatctggcctctctgaatattgcccgtttttcgacgaccccgcctgacgcgatcgataaattactcaaagatgctgaagaacgtaatttgagccttctgtccgcgcgcctgggccaagacctcgcgcgtgaaaacattcgccttgcgcaatcaggccatctgccaaccgtcaatctgaatgcgtctaccggcgttagtaacagccacaatcatgggtctgcgttaccgccggaaacgccggccaactcacgtaacagttacaatggccaaagctccatcggtttatcgctgagcattcctctgtacacgggtggtcgtaccagctcgcaggtcgagcaagctcagtacggttttaccagtgcatcggaacagcttgaatcggtgtatcgcagcattgttcagattgcgcgcagtagctataataatattagtgcgagcatctcctcgatcaaagcatatcaacaagtcgtcgtgtccgcgcaaagtagcttagacgcaacagaagcgggctaccaggtcgggacccgcactatcgtcgatgtattaaacgcgaccaccaccctgtacgatgcaaaacagaaactgtcgtcagcacgttatgattaccttattaatcaacttaacattcaatacgctcgcggcacgctgaacgaaaatgacctgatccagctgaataacgctctgggtgaggagatctcgaccagcccagataacattatccgtccccttacgagcccggttctgaatactaccaattaa BBa_K1406000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z