BBa_K1683003 1 BBa_K1683003 E. coli tolC signaling sequence + Proteus mirabilis tolC 2015-09-16T11:00:00Z 2015-09-16T11:48:31Z The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Proteus mirabilis. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen BBa_K1683003_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgaagaccttttgcaagtctaccagaaagcaaaagacagcaacccggagttgcgtaaatcacttgcggaacgcaatcaggcgttcgaaaaaattaatgaagcccgctctccgctgctgcctcagctcggcctgggagcctcaattgattataaaagtggttaccgtgatgccaaaaataccgagtctaattcgctgggagcgaacctgacactgacccaaaccatctttgacatgagcctgtggcgccagcttaccatgcaagagaaaacagcgggcatgagcgatgttacctatcaaacatctcaacagcaattaattctggataccgcgactgcgtactttaatgtgctgcgtgcgattgatagcctgagtttcattgaggcgcagaaagaacaggtctatcgccagctggaccaaacgacgcagcgctttaatgttggtctggtcgcaattactgatgtgcaaaacgctcgtgcgaattatgacagcgtgctggcacaagaagtggcaggtcgtaatgagctggacaacgcactggaaaaactccgccaggtgtcgggggtttattacattaatctggcctctctgaatattgcccgtttttcgacgaccccgcctgacgcgatcgataaattactcaaagatgctgaagaacgtaatttgagccttctgtccgcgcgcctgggccaagacctcgcgcgtgaaaacattcgccttgcgcaatcaggccatctgccaaccgtcaatctgaatgcgtctaccggcgttagtaacagccacaatcatgggtctgcgttaccgccggaaacgccggccaactcacgtaacagttacaatggccaaagctccatcggtttatcgctgagcattcctctgtacacgggtggtcgtaccagctcgcaggtcgagcaagctcagtacggttttaccagtgcatcggaacagcttgaatcggtgtatcgcagcattgttcagattgcgcgcagtagctataataatattagtgcgagcatctcctcgatcaaagcatatcaacaagtcgtcgtgtccgcgcaaagtagcttagacgcaacagaagcgggctaccaggtcgggacccgcactatcgtcgatgtattaaacgcgaccaccaccctgtacgatgcaaaacagaaactgtcgtcagcacgttatgattaccttattaatcaacttaacattcaatacgctcgcggcacgctgaacgaaaatgacctgatccagctgaataacgctctgggtgaggagatctcgaccagcccagataacattatccgtccccttacgagcccggttctgaatactaccaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z