BBa_K1683004 1 BBa_K1683004 pBAD+Strong RBS+E. coli tolC signal sequence+P. aeruginosa oprM 2015-09-16T11:00:00Z 2015-09-18T03:48:02Z BBa_K1406000 comes from the biobrick registry The tolC signaling sequence and Pseudomonas aeruginosa PA01 tolC sequence were synthesized from DNA sequences found in genomic databases. Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Pseudomonas aeruginosa PA01 tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Pseudomonas aeruginosa PA01. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen component2464259 1 BBa_K1406000 component2464260 1 BBa_K1683005 annotation2464260 1 BBa_K1683005 range2464260 1 152 1624 annotation2464259 1 BBa_K1406000 range2464259 1 1 151 BBa_K1406000 1 BBa_K1406000 pBAD promoter + strong RBS 2014-10-08T11:00:00Z 2015-05-08T01:10:17Z E. coli A combinatorial part of K206000, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pBAD promoter and the strong RBS using the restriction enzymes NheI and PstI. false false _1784_ 0 17066 9 It's complicated false We say we are hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick. false Sierra Tackett annotation2408505 1 AraI2 range2408505 1 61 78 annotation2408503 1 promoter range2408503 1 1 131 annotation2408504 1 AraI1 range2408504 1 40 57 annotation2408508 1 BBa_B0034 range2408508 1 139 150 annotation2408506 1 BBa_K206000 range2408506 1 1 130 annotation2408507 1 conserved range2408507 1 143 146 BBa_K1683005 1 BBa_K1683005 E. coli tolC signaling sequence + Pseudomonas aeruginosa oprM 2015-09-16T11:00:00Z 2015-09-18T03:47:46Z The tolC signaling sequence and Pseudomonas aeruginosa PA01 tolC sequence were synthesized from DNA sequences found in genomic databases. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Pseudomonas aeruginosa PA01. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. false false _2101_ 18345 18345 9 false The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible. false Matthew W Mortensen BBa_K1683004_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagcatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggcctgtagtctcatcccggattatcagcgcccggaagcaccggtagccgcagcgtacccgcagggtcaggcctacggtcaaaatacaggggcggcagctgttccggccgcggatattggttggcgcgaattttttcgcgatccgcagctgcaacagctgatcggcgtcgccttggaaaataatcgcgacctccgtgtcgctgccttaaacgtcgaagcttttcgcgcgcaataccgcatccaacgcgcggatctctttcctcgtattggcgtggacgggagcgggacccgtcagcgtcttcccggcgacctttctacaaccggctcaccagccatttcctctcaatatggcgttaccctggggacaaccgcgtgggaactcgacctgttcgggcgcctgcgtagtcttcgtgaccaggcgctggaacagtatctggcgaccgaacaggcccagcgctcagcgcagaccaccttagtggcgtcggtcgcaacggcttatctgacactgaaagccgaccaagctcaacttcagttgacgaaagatacgctgggcacttatcagaaatcctttgatttgacccaacgcagctatgatgtgggggtggcgtctgcactggatttgcgtcaggcccaaactgctgttgaaggcgcgcgcgccacgttggcacagtatacccgtcttgtagcacaggatcagaacgccctggtgctgctgctgggctcaggtattcccgcaaacctgccgcaaggcttgggcttggatcagacgctgctgaccgaggtgcccgcaggcctgccgtctgatctgcttcagcgccgtccggacatcctggaagccgaacaccaactgatggcggctaatgcgtcgattggggcagcgcgcgcagcgttcttcccctctatttcgctgaccgctaacgctggcaccatgtcgcgtcagttgagcggtcttttcgacgcgggatcgggtagctggctgttccagccatctattaacctgccgatctttacggcaggctcgttgcgtgcatcgcttgattacgcaaaaatccagaaagacatcaatgtggcacagtatgagaaagcgatccagacagcgtttcaagaagttgctgatggcctggcagcgcgcggcacgttcaccgaacagctacaggcccagcgcgacctggtcaaagcatctgacgaatactatcagttagctgataagcgctaccgcactggtgtggataattacctgactctgttagatgcccagcgttccctgtttaccgctcagcaacagctgatcaccgatcgtctgaatcagctgacctcggaagtgaatctgtataaagcgttgggtggggggtggaaccagcaaaccgtaacccagcaacagacggctaaaaaagaagatccgcaggcgtga BBa_K1683005_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggcctgtagtctcatcccggattatcagcgcccggaagcaccggtagccgcagcgtacccgcagggtcaggcctacggtcaaaatacaggggcggcagctgttccggccgcggatattggttggcgcgaattttttcgcgatccgcagctgcaacagctgatcggcgtcgccttggaaaataatcgcgacctccgtgtcgctgccttaaacgtcgaagcttttcgcgcgcaataccgcatccaacgcgcggatctctttcctcgtattggcgtggacgggagcgggacccgtcagcgtcttcccggcgacctttctacaaccggctcaccagccatttcctctcaatatggcgttaccctggggacaaccgcgtgggaactcgacctgttcgggcgcctgcgtagtcttcgtgaccaggcgctggaacagtatctggcgaccgaacaggcccagcgctcagcgcagaccaccttagtggcgtcggtcgcaacggcttatctgacactgaaagccgaccaagctcaacttcagttgacgaaagatacgctgggcacttatcagaaatcctttgatttgacccaacgcagctatgatgtgggggtggcgtctgcactggatttgcgtcaggcccaaactgctgttgaaggcgcgcgcgccacgttggcacagtatacccgtcttgtagcacaggatcagaacgccctggtgctgctgctgggctcaggtattcccgcaaacctgccgcaaggcttgggcttggatcagacgctgctgaccgaggtgcccgcaggcctgccgtctgatctgcttcagcgccgtccggacatcctggaagccgaacaccaactgatggcggctaatgcgtcgattggggcagcgcgcgcagcgttcttcccctctatttcgctgaccgctaacgctggcaccatgtcgcgtcagttgagcggtcttttcgacgcgggatcgggtagctggctgttccagccatctattaacctgccgatctttacggcaggctcgttgcgtgcatcgcttgattacgcaaaaatccagaaagacatcaatgtggcacagtatgagaaagcgatccagacagcgtttcaagaagttgctgatggcctggcagcgcgcggcacgttcaccgaacagctacaggcccagcgcgacctggtcaaagcatctgacgaatactatcagttagctgataagcgctaccgcactggtgtggataattacctgactctgttagatgcccagcgttccctgtttaccgctcagcaacagctgatcaccgatcgtctgaatcagctgacctcggaagtgaatctgtataaagcgttgggtggggggtggaaccagcaaaccgtaacccagcaacagacggctaaaaaagaagatccgcaggcgtga BBa_K1406000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z