BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1685001 1 BBa_K1685001 aeBlue+TT 2015-09-15T11:00:00Z 2015-09-18T03:30:13Z The sequence was based on BBa_K864401 by Uppsala 2012 and the codon-optimised. This part encodes a chromoprotein, aeBlue, with a terminator. This part can be assembled to make a reporter. false false _2103_ 25100 25100 9 false Codon optimisation was done using IDT tool (http://sg.idtdna.com/CodonOpt). false ITB_Indonesia 2015 component2461230 1 BBa_B0015 component2461223 1 BBa_K1685005 annotation2461223 1 BBa_K1685005 range2461223 1 1 699 annotation2461230 1 BBa_B0015 range2461230 1 708 836 BBa_K1685005 1 BBa_K1685005 aeBlue 2015-09-15T11:00:00Z 2016-01-25T01:09:54Z The aeBlue sequence from Uppsala team 2012 (BBa_K864401) was chemically synthesised. This part encodes a blue chromoprotein named aeBlue. This part is useful for reporter. false false _2103_ 4206 25100 9 false The part has been codon-optimised for expression in E. coli using http://sg.idtdna.com/CodonOpt false ITB_Indonesia 2015 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1685005_sequence 1 atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcactaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1685001_sequence 1 atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z