BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K1685002
1
BBa_K1685002
aeBlue with LVA tag and double terminator
2015-09-15T11:00:00Z
2015-09-27T05:45:33Z
The part was chemically synthesised.
This part encodes a chromoprotein, aeBlue, with LVA degradation tag dan double terminator
false
false
_2103_
4206
25100
9
false
The aeBlue coding sequence was codon-optimised.
false
ITB_Indonesia 2015
component2477826
1
BBa_B0010
component2477828
1
BBa_B0012
component2477825
1
BBa_K1685006
annotation2477828
1
BBa_B0012
range2477828
1
829
869
annotation2477826
1
BBa_B0010
range2477826
1
741
820
annotation2477825
1
BBa_K1685006
range2477825
1
1
732
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1685006
1
BBa_K1685006
aeBlue with LVA tag
2015-09-15T11:00:00Z
2015-09-27T05:52:46Z
The BBa_K1685005 part is given LVA-degradation tag designed by Edinburgh 2014 team.
This part encodes a chromoprotein, aeBlue, with LVA degradation tag
false
false
_2103_
4206
25100
9
false
The aeBlue sequence was codon-optimised using http://sg.idtdna.com/CodonOpt
false
ITB_Indonesia 2015
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1685002_sequence
1
atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcacgctgcaaacgacgaaaactacgctctggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1685006_sequence
1
atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcacgctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z