LOCUS       BBa_K1685009            1284 bp    DNA     linear       26-Dec-2024
DEFINITION  Strongly & constitutively expressed LacI
ACCESSION   BBa_K1685009
VERSION     BBa_K1685009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1147
                     /label=BBa_I732100
     stem_loop       1167..1210
                     /label=stem_loop
     misc_feature    1156..1235
                     /label=BBa_B0010
     terminator      1156..1235
                     /label=BBa_B0010
     stem_loop       1251..1270
                     /label=T7 TE
     misc_feature    1277..1277
                     /label=stop
     polyA_site      1271..1284
                     /label=polya
     misc_feature    1244..1284
                     /label=BBa_B0012
     terminator      1244..1284
                     /label=BBa_B0012
     misc_feature    44..1284
                     /label=BBa_I732820
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
      121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
      781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
      841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
      901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
      961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1261 cgggtgggcc tttctgcgtt tata
//