LOCUS       BBa_K1685011            2573 bp    DNA     linear       05-May-2025
DEFINITION  Rhamnolipid production
ACCESSION   BBa_K1685011
VERSION     BBa_K1685011.1  
FEATURES             Location/Qualifiers
     promoter        30..52
                     /label=T7 promoter
     misc_feature    55..75
                     /label=lac operator
     RBS             107..112
                     /label=RBS
     promoter        1..114
                     /label=BBa_K613010
     misc_feature    123..2436
                     /label= BBa_ K653000 RhlAB Panama
     misc_feature    123..141
                     /label=Primer
     misc_feature    157..2356
                     /label=Rhamnosyltransferase gene complex 1
     CDS             157..1044
                     /label=Rhamnosyltranferase A
     misc_feature    876..881
                     /label=Silent Mutation
     CDS             1110..2390
                     /label=Rhamnosyltransferase B
     misc_feature    1899..1904
                     /label=Silent Mutation
     misc_feature    2013..2018
                     /label=Silent Mutation
     misc_feature    2418..2436
                     /label=Primer
     CDS             123..2436
                     /label=BBa_K653000
     stem_loop       2456..2499
                     /label=stem_loop
     misc_feature    2445..2524
                     /label=BBa_B0010
     terminator      2445..2524
                     /label=BBa_B0010
     stem_loop       2540..2559
                     /label=T7 TE
     misc_feature    2566..2566
                     /label=stop
     polyA_site      2560..2573
                     /label=polya
     misc_feature    2533..2573
                     /label=BBa_B0012
     terminator      2533..2573
                     /label=BBa_B0012
     terminator      2445..2573
                     /label=BBa_B0015
ORIGIN
        1 gaagcgagag gatcttaagg ctagagtacc aatacgactc actataggga gaggaattgt
       61 gagcggataa caattcccac tagaaataat tttgtttaac ttaagaagga ggaatactag
      121 aggtttgcct gttcgaaaat ttttgggagg tgtgaaatgc ggcgcgaaag tctgttggta
      181 tcggtttgca agggcctgcg ggtacatgtc gagcgcgttg ggcaggatcc cgggcgcagc
      241 acggtgatgc tggtcaacgg cgcgatggcg accaccgcct cgttcgcccg gacctgcaag
      301 tgcctggccg aacatttcaa cgtggtgctg ttcgacctgc ccttcgccgg gcagtcgcgt
      361 cagcacaacc cgcagcgcgg gttgatcacc aaggacgacg aggtggaaat cctcctggcg
      421 ctgatcgagc gcttcgaggt caatcacctg gtctccgcgt cctggggcgg tatctccacg
      481 ctgctggcgc tgtcgcgcaa tccgcgcggc atccgcagct cggtggtgat ggcattcgcc
      541 cctggactga accaggcgat gctcgactac gtcgggcggg cgcaggcgct gatcgagctg
      601 gacgacaagt cggcgatcgg ccatctgctc aacgagaccg tcggcaaata cctgccgccg
      661 cgcctgaaag ccagcaacca tcagcacatg gcttcgctgg ccaccggcga atacgagcag
      721 gcgcgctttc acatcgacca ggtgctggcg ctcaacgatc ggggctacct ggcttgcctg
      781 gagcggatcc agagccacgt gcatttcatc aacggcagct gggacgaata caccaccgcc
      841 gaggacgccc gccagttccg cgactacctg ccgcactgta gtttctcgcg ggtggagggc
      901 accgggcatt tcctcgacct ggagtccaag ctggccgcgg tacgcgtgca ccgcgccctg
      961 ctcgagcacc tgctgaagca accggagccg cagcgggcgg aacgcgcggc gggattccac
     1021 gagatggcca tcggctacgc ctgaaccctt gacctgcgaa gacccggcct ggccgggctt
     1081 tgcggttgca taacgcacgg agtagcccca tgcacgccat cctcatcgcc atcggctcgg
     1141 ccggcgacgt atttcccttc atcggcttgg cccggaccct gaaattgcgc gggcaccgcg
     1201 tgagcctctg caccatcccg gtgtttcgcg acgcggtgga gcagcacggc atcgcgttcg
     1261 tcccgctgag cgacgaactg acctaccgcc gggccatggg cgatccgcgc ctgtgggacc
     1321 ccaagacgtc cttcggcgtg ctctggcaag ccatcgccgg gatgatcgag ccggtctacg
     1381 agtacgtctc ggcgcagcgc catgacgaca tcgtggtggt cggctcgctc tgggcgctgg
     1441 gcgcacgcat cgctcacgag aagtacggga ttccctacct gtccgcgcag gtctcgccat
     1501 cgaccttgct gtcggcgcac ctgccgccgg tacaccccaa gttcaacgtg cccgagcaga
     1561 tgccgctggc gatgcgcaag ctgctctggc gctgcatcga gcgcttcaag ctggatcgca
     1621 cctgcgcgcc ggagatcaac gcggtgcgcc gcaaggtcgg cctggagacg ccggtgaagc
     1681 gcatcttcac ccaatggatg cattcgccgc agggcgtggt ctgcctgttc ccggcctggt
     1741 tcgcgccacc ccagcaggat tggccgcaac ccctgcacat gaccggcttc ccgctgttcg
     1801 acggcagtat cccggggacc ccgctcgacg acgaactgca acgctttctc gatcagggca
     1861 gccggccgct ggtgttcacc cagggctcga ccgaacacct acagggcgac ttctacgcca
     1921 tggccctgcg cgcgctggaa cgcctcggcg cgcgtgggat cttcctcacc ggcgccggcc
     1981 aggaaccgct gcgcggcttg ccgaatcacg tgctacagcg cgcctacgcg ccactgggag
     2041 ccttgctgcc atcgtgcgcc gggctggtcc atccgggcgg tatcggcgcc atgagcctgg
     2101 ccttggcggc gggggtgccg caggtgctgc tgccctgcgc ccacgaccag ttcgacaatg
     2161 ccgaacggct ggtccggctc ggctgcggga tgcgcctggg cgtgccgttg cgcgagcagg
     2221 agttgcgcgg ggcgctgtgg cgcttgctcg aggacccggc catggcggcg gcctgtcggc
     2281 gtttcatgga attgtcacaa ccgcacagta tcgcttgcgg taaagcggcc caggtggtcg
     2341 aacgttgtca tagggagggg gatgcgcgat ggctgaaggc tgcgtcctga acggtgctgg
     2401 cataacagat agggttgcca tgattttgcc gtatcgtact agagccaggc atcaaataaa
     2461 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2521 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//