BBa_K1685011
type: ComponentDefinition
persistent identity: BBa K1685011
display id: BBa_K1685011
version: 1
was generated by: iGEM to SBOL conversion
description: Rhamnolipid production
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T09:45:52Z
mutable provenance: The part was chemically synthesised. The sequences were based on parts available in Registry.
owned by: james
owned by: myers
mutable description: This part encodes two enzymes, rhlA and rhlB (BBa_K653000), for biosynthesis of rhamnolipid. The enzymes are under T7 promoter with medium strengh and a strong ribosomal binding site (BBa_K613010), and stopped with a double terminator (BBa_B0015). The promoter had the second highest efficiency as shown by EPFL team 2011 (http://parts.igem.org/Part:BBa_K613010:Experience).
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 25100
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We include the scar sequences between RBS-coding and coding-terminator in hope that it will function properly.
star: false
creator: ITB_Indonesia 2015
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K613010
component: BBa_B0015
component: BBa_K653000
sequence annotation: BBa_K613010
sequence annotation: BBa_K653000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1685011_sequence/1

BBa_K613010
type: Component
persistent identity: component2461648
display id: component2461648
version: 1
top level: BBa_K1685011
definition: BBa_K613010
access: public

BBa_B0015
type: Component
persistent identity: component2461665
display id: component2461665
version: 1
top level: BBa_K1685011
definition: BBa_B0015
access: public

BBa_K653000
type: Component
persistent identity: component2461658
display id: component2461658
version: 1
top level: BBa_K1685011
definition: Biosurfact
access: public

BBa_K613010
type: SequenceAnnotation
persistent identity: annotation2461648
display id: annotation2461648
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461648/range2461648/1
component: BBa_K613010

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461648/range2461648/1
type: Range
persistent identity: range2461648
display id: range2461648
version: 1
top level: BBa_K1685011
direction: direction/reverse
start: 1
end: 114
orientation: inline

BBa_K653000
type: SequenceAnnotation
persistent identity: annotation2461658
display id: annotation2461658
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461658/range2461658/1
component: BBa_K653000

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461658/range2461658/1
type: Range
persistent identity: range2461658
display id: range2461658
version: 1
top level: BBa_K1685011
direction: direction/reverse
start: 123
end: 2436
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2461665
display id: annotation2461665
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461665/range2461665/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461665/range2461665/1
type: Range
persistent identity: range2461665
display id: range2461665
version: 1
top level: BBa_K1685011
direction: direction/reverse
start: 2445
end: 2573
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K613010
type: ComponentDefinition
persistent identity: BBa K613010
display id: BBa_K613010
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter family member (LacI repressed)
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:54Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.
discontinued: false
dominant: false
experience: experience/None
group u list: _785_
m user id: 0
owner id: 7125
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).
star: false
creator: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K613010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2136238
display id: annotation2136238
version: 1
top level: BBa_K613010
location: https://synbiohub.org/public/igem/BBa_K613010/annotation2136238/range2136238/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K613010/annotation2136238/range2136238/1
type: Range
persistent identity: range2136238
display id: range2136238
version: 1
top level: BBa_K613010
direction: direction/forward
start: 30
end: 52
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2136239
display id: annotation2136239
version: 1
top level: BBa_K613010
location: https://synbiohub.org/public/igem/BBa_K613010/annotation2136239/range2136239/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K613010/annotation2136239/range2136239/1
type: Range
persistent identity: range2136239
display id: range2136239
version: 1
top level: BBa_K613010
direction: direction/forward
start: 55
end: 75
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136240
display id: annotation2136240
version: 1
top level: BBa_K613010
location: https://synbiohub.org/public/igem/BBa_K613010/annotation2136240/range2136240/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K613010/annotation2136240/range2136240/1
type: Range
persistent identity: range2136240
display id: range2136240
version: 1
top level: BBa_K613010
direction: direction/forward
start: 107
end: 112
orientation: inline

Biosurfact
type: ComponentDefinition
persistent identity: BBa K653000
display id: BBa_K653000
version: 1
was generated by: iGEM to SBOL conversion
description: Re-designing "The Biosurfactator" The first Central American BioBrick
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
mutable provenance: Pseudomonas aeruginosa from INDICASAT lab. and the genomic sequence of RhlAB was reviwed in genBank (ID 878955 and 878954) http://www.ncbi.nlm.nih.gov/nuccore/L28170.1
owned by: james
owned by: myers
mutable description: Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (RhlAB). This is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid, which is a biomolecule with surfactant properties, but the natural RhlAB gene has illegal restriction sites that make it incompatible with the Assembly Standard Protocol 10, specifically the Pstl1 restriction sites. The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year???s iGEM project we failed in producing the rhamnolipid compound into our E. coli based-factory, because our last year???s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part BBa_K424018 (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone. As scientist and iGEMers, we have the responsibility to deliver a functional BioBrick, thus according to iGEM???s competition new slogan, ???Quality not Quantity???, we have embraced the challenge of re-designing and re-building the same BioBrick part from last year in order to comply this new standard based on quality. How to use it as a BioBrick? : We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
discontinued: false
dominant: false
experience: experience/None
group u list: _545_830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The RhlAB gene complex has shown illegal restriction sites according to the assembly standard protocol 10. We faced this problem by applying the mutagenesis protocol that consist in doing silent mutations that change the nucleotides of our rhlAB gene that are cut by the Pstl restriction enzyme. This will give us a standardized coding sequence of the rhlAB gene compatible with the Assembly Standard protocol 10 and a new BioBrick part for the Registry.
star: false
creator: Team Panama 2011
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_ K653000 RhlAB Panama
sequence annotation: Primer
sequence annotation: Rhamnosyltransferase gene complex 1
sequence annotation: Rhamnosyltranferase A
sequence annotation: Silent Mutation
sequence annotation: Rhamnosyltransferase B
sequence: https://synbiohub.org/public/igem/BBa_K653000_sequence/1

BBa_ K653000 RhlAB Panama
type: SequenceAnnotation
persistent identity: annotation2142961
display id: annotation2142961
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1
type: Range
persistent identity: range2142961
display id: range2142961
version: 1
top level: Biosurfact
direction: direction/forward
start: 1
end: 2314
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142962
display id: annotation2142962
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1
type: Range
persistent identity: range2142962
display id: range2142962
version: 1
top level: Biosurfact
direction: direction/forward
start: 1
end: 19
orientation: inline

Rhamnosyltransferase gene complex 1
type: SequenceAnnotation
persistent identity: annotation2142963
display id: annotation2142963
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1
type: Range
persistent identity: range2142963
display id: range2142963
version: 1
top level: Biosurfact
direction: direction/forward
start: 35
end: 2234
orientation: inline

Rhamnosyltranferase A
type: SequenceAnnotation
persistent identity: annotation2142964
display id: annotation2142964
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1
type: Range
persistent identity: range2142964
display id: range2142964
version: 1
top level: Biosurfact
direction: direction/forward
start: 35
end: 922
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142965
display id: annotation2142965
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1
type: Range
persistent identity: range2142965
display id: range2142965
version: 1
top level: Biosurfact
direction: direction/forward
start: 754
end: 759
orientation: inline

Rhamnosyltransferase B
type: SequenceAnnotation
persistent identity: annotation2142966
display id: annotation2142966
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1
type: Range
persistent identity: range2142966
display id: range2142966
version: 1
top level: Biosurfact
direction: direction/forward
start: 988
end: 2268
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142967
display id: annotation2142967
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1
type: Range
persistent identity: range2142967
display id: range2142967
version: 1
top level: Biosurfact
direction: direction/forward
start: 1777
end: 1782
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142968
display id: annotation2142968
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1
type: Range
persistent identity: range2142968
display id: range2142968
version: 1
top level: Biosurfact
direction: direction/forward
start: 1891
end: 1896
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142969
display id: annotation2142969
version: 1
top level: Biosurfact
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1
type: Range
persistent identity: range2142969
display id: range2142969
version: 1
top level: Biosurfact
direction: direction/reverse
start: 2296
end: 2314
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1685011_sequence/1
type: Sequence
persistent identity: BBa K1685011 sequence
display id: BBa_K1685011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagcgagaggatcttaaggctagagtaccaatacgactcactatagggagaggaattgtgagcggataacaattcccactagaaataattttgtttaacttaagaaggaggaatactagaggtttgcctgttcgaaaatttttgggaggtgtgaaatgcggcgcgaaagtctgttggtatcggtttgcaagggcctgcgggtacatgtcgagcgcgttgggcaggatcccgggcgcagcacggtgatgctggtcaacggcgcgatggcgaccaccgcctcgttcgcccggacctgcaagtgcctggccgaacatttcaacgtggtgctgttcgacctgcccttcgccgggcagtcgcgtcagcacaacccgcagcgcgggttgatcaccaaggacgacgaggtggaaatcctcctggcgctgatcgagcgcttcgaggtcaatcacctggtctccgcgtcctggggcggtatctccacgctgctggcgctgtcgcgcaatccgcgcggcatccgcagctcggtggtgatggcattcgcccctggactgaaccaggcgatgctcgactacgtcgggcgggcgcaggcgctgatcgagctggacgacaagtcggcgatcggccatctgctcaacgagaccgtcggcaaatacctgccgccgcgcctgaaagccagcaaccatcagcacatggcttcgctggccaccggcgaatacgagcaggcgcgctttcacatcgaccaggtgctggcgctcaacgatcggggctacctggcttgcctggagcggatccagagccacgtgcatttcatcaacggcagctgggacgaatacaccaccgccgaggacgcccgccagttccgcgactacctgccgcactgtagtttctcgcgggtggagggcaccgggcatttcctcgacctggagtccaagctggccgcggtacgcgtgcaccgcgccctgctcgagcacctgctgaagcaaccggagccgcagcgggcggaacgcgcggcgggattccacgagatggccatcggctacgcctgaacccttgacctgcgaagacccggcctggccgggctttgcggttgcataacgcacggagtagccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcttggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccgggccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggagacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccggggaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctacagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K613010_sequence/1
type: Sequence
persistent identity: BBa K613010 sequence
display id: BBa_K613010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagcgagaggatcttaaggctagagtaccaatacgactcactatagggagaggaattgtgagcggataacaattcccactagaaataattttgtttaacttaagaaggaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K653000_sequence/1
type: Sequence
persistent identity: BBa K653000 sequence
display id: BBa_K653000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttgcctgttcgaaaatttttgggaggtgtgaaatgcggcgcgaaagtctgttggtatcggtttgcaagggcctgcgggtacatgtcgagcgcgttgggcaggatcccgggcgcagcacggtgatgctggtcaacggcgcgatggcgaccaccgcctcgttcgcccggacctgcaagtgcctggccgaacatttcaacgtggtgctgttcgacctgcccttcgccgggcagtcgcgtcagcacaacccgcagcgcgggttgatcaccaaggacgacgaggtggaaatcctcctggcgctgatcgagcgcttcgaggtcaatcacctggtctccgcgtcctggggcggtatctccacgctgctggcgctgtcgcgcaatccgcgcggcatccgcagctcggtggtgatggcattcgcccctggactgaaccaggcgatgctcgactacgtcgggcgggcgcaggcgctgatcgagctggacgacaagtcggcgatcggccatctgctcaacgagaccgtcggcaaatacctgccgccgcgcctgaaagccagcaaccatcagcacatggcttcgctggccaccggcgaatacgagcaggcgcgctttcacatcgaccaggtgctggcgctcaacgatcggggctacctggcttgcctggagcggatccagagccacgtgcatttcatcaacggcagctgggacgaatacaccaccgccgaggacgcccgccagttccgcgactacctgccgcactgtagtttctcgcgggtggagggcaccgggcatttcctcgacctggagtccaagctggccgcggtacgcgtgcaccgcgccctgctcgagcacctgctgaagcaaccggagccgcagcgggcggaacgcgcggcgggattccacgagatggccatcggctacgcctgaacccttgacctgcgaagacccggcctggccgggctttgcggttgcataacgcacggagtagccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcttggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccgggccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggagacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccggggaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctacagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttgccgaatcacgtgctacagcgcgcctacgcgccactgggagccttgctgccatcgtgcgccgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z