BBa_J45992 1 p(osmY) Full-length stationary phase osmY promoter 2006-07-10T11:00:00Z 2015-08-31T04:08:50Z This part was PCRed out of E. coli's genome. osmY's promoter is active in stationary phase and under high osmotic pressure conditions. false false _84_ 0 642 84 Not in stock true Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol false Stephen Payne annotation1883645 1 -10 range1883645 1 188 193 annotation1883646 1 -35 range1883646 1 164 169 BBa_K1686046 1 BBa_K1686046 Curdlan synthase gene with codon optimisation for E. coli 2015-09-05T11:00:00Z 2015-09-12T08:06:56Z Curdlan and other bacterial (1→3)-β-D-glucans. M. McIntosh . B. A. Stone . V. A. Stanisich - Appl Microbiol Biotechnol (2005) 68: 163???173; DOI 10.1007/s00253-005-1959-5 (Karnezis et al. 2000, 2003) The curdlan synthase (73 kDa) coded by crdS (1,965 bp) polymerizes UDP glucose into beta 1,3 D Glucan. CrdS shares no homology with the (1→3)- β-glucan synthase-related FSK1 and FSK2 proteins from fungi (e.g. Saccharomyces, Candida, Aspergillus; Dijkgraaf et al. 2001) or the plant callose synthase-related proteins (Li et al. 2003), which are both classified as GT48 glycosyltransferases. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout???Cin disposition). In Agrobacterium, this gene is flancked by two other genes (crdA and crdC) who's corresponding proteins help transport the nascent polymer across the cytoplasmic membrane and into the periplasm. The process of polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane and so is unlikely to involve the periplasmic CrdC, or CrdA directly. false false _2104_ 25369 25369 9 false The gene sequence that was used was found in the publication written below. We then verified that the EcoRI, XbaI, SpeI, PstI sequences were not in the gene which was the case. Finally we optimized the sequence using IDT's optimization software on the internet for E.coli. GenBank : AF057142.2 Publication : ????Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene???? , Stan J.Stasinopoulos & all, 1999 false Jean Descarpentrie annotation2445184 1 Stop codon range2445184 1 1963 1965 annotation2445183 1 Start codon range2445183 1 1 3 BBa_K1686033 1 BBa_K1686033 Curdlan synthase under control of stationary phase promoter 2015-09-11T11:00:00Z 2015-09-27T05:04:14Z kkk kkk false false _2104_ 25368 25369 9 false kkk false Jean Descarpentrie component2452736 1 BBa_J45992 component2452739 1 BBa_K1686046 annotation2452739 1 BBa_K1686046 range2452739 1 206 2170 annotation2452736 1 BBa_J45992 range2452736 1 1 199 BBa_J45992_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa BBa_K1686033_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagatgtatttcagtgctgaaggtgatgtacagagtgtgttatacgtgaacctgacgattgcaatcggtgcaatcctgtttgccctgctggctgatccgcgtaagatggttgatcgcctggcattcagcatcatcatgttgttatctctgggtgtctatattgtctggcgtgccacggacaccttgcctccacctgaactcagcctcgaaacgctgtggtgctatacttatttcaccttcgagctgatcagtgtactgtatgctatgggctcaattctcattttactgcgtcggaccgattggagtgcggtggccgatcagggagaagcctatttagctggcaatccacacgccccgttagtggacgtttttatttgcacctataatgaacctttgaacgtgctcgagaaatcgattattgcggcacaagctatggactatccgcgcttgcgcgtgttcgtgtgtgacgatacccggcgtggggaggtccgcacctactgcgaggcggtgggggtcaactatgtaacccgtcctgataataaacatgctaaagctggcaacttgaataacgctttactgcacaccaacgcgttagaggaagtaagtgactttattatggttctggatgctgactttgcaccacaggcaaacttcctgcgccgcgtcacaggtttgttctcggatcctaaagtcgcggtagtccagactccgcagttttattttaattcagatccaatccagcacaacctgggaatcgataaaagcttcgtagatgatcaacgtgttttttttgacgtgttccagccggcgaaagatgccgtcggttgtgcgttctgcgttgggaccagctttgtggtccgccgggcagccgtaaatgggattgggggctttccttcggacgcgttgtctgaagacatgttgctgacataccgcttaatggaacgtggctacgtgacccgttggctcaatgaaaaactgagtgttggtttaagtgctgaaggagtacctgaatatattacccagcgcactcgttggtgtctgggcactatccagatcggcctgttacgtacgggccccctgtggcgtggcaattttacccttactcagcggctgcattaccttcatggcctgttctgttggctctccaaaccgtttattctgtgtctgctgcttgcgccgagcatctattggctgaccggtgtgagtgcgttgcaggtcgatgaactgatgtttatgaaattaggcttatcgagtctggcactgttttggacctattcgacatggattagcggcaaacgcacccttccgttgttcacggaggttacacatgcgctgacagctgtaccgattacgattactctgttccaggccatccgtaagccgttcggccgcccgttcaaagtaacggaaaaaggtggcgatcgttcccaggttcgcgtgcatttgccgactgctatcttttttgcctttgtcacgttatccagtgcggtgagcatcgttctggccgtatacggcctggatgcgcctagcgaactgtcttctcgtgattgcttaaacctgatctggtcggccgtcgccatggtgattgcattcacgtcatttatttgttgcattgaattgcctcgctttggaaaagaggagatgattggtgtcgatttccgcggtcagcttcgttccgcgtcgtcgactcgcccggtacggatcaccggcctgtcgaccgaaaacattacgctggcggcggtaccgtccagttcggatgttaaagatgtgttcgtgccggaggcgggatggatgcgcatcagtccagcggaacatgcgcagaactcgggcaaattcgacatccacccgtctgacgaacagcgtcgctctattctgcgcttattgtttcgcaaggccccggaaaatgtggcggagcaaggtgacttaatgaaaagtatgcgtattcttcttgcacgtgccttcggctga BBa_K1686046_sequence 1 atgtatttcagtgctgaaggtgatgtacagagtgtgttatacgtgaacctgacgattgcaatcggtgcaatcctgtttgccctgctggctgatccgcgtaagatggttgatcgcctggcattcagcatcatcatgttgttatctctgggtgtctatattgtctggcgtgccacggacaccttgcctccacctgaactcagcctcgaaacgctgtggtgctatacttatttcaccttcgagctgatcagtgtactgtatgctatgggctcaattctcattttactgcgtcggaccgattggagtgcggtggccgatcagggagaagcctatttagctggcaatccacacgccccgttagtggacgtttttatttgcacctataatgaacctttgaacgtgctcgagaaatcgattattgcggcacaagctatggactatccgcgcttgcgcgtgttcgtgtgtgacgatacccggcgtggggaggtccgcacctactgcgaggcggtgggggtcaactatgtaacccgtcctgataataaacatgctaaagctggcaacttgaataacgctttactgcacaccaacgcgttagaggaagtaagtgactttattatggttctggatgctgactttgcaccacaggcaaacttcctgcgccgcgtcacaggtttgttctcggatcctaaagtcgcggtagtccagactccgcagttttattttaattcagatccaatccagcacaacctgggaatcgataaaagcttcgtagatgatcaacgtgttttttttgacgtgttccagccggcgaaagatgccgtcggttgtgcgttctgcgttgggaccagctttgtggtccgccgggcagccgtaaatgggattgggggctttccttcggacgcgttgtctgaagacatgttgctgacataccgcttaatggaacgtggctacgtgacccgttggctcaatgaaaaactgagtgttggtttaagtgctgaaggagtacctgaatatattacccagcgcactcgttggtgtctgggcactatccagatcggcctgttacgtacgggccccctgtggcgtggcaattttacccttactcagcggctgcattaccttcatggcctgttctgttggctctccaaaccgtttattctgtgtctgctgcttgcgccgagcatctattggctgaccggtgtgagtgcgttgcaggtcgatgaactgatgtttatgaaattaggcttatcgagtctggcactgttttggacctattcgacatggattagcggcaaacgcacccttccgttgttcacggaggttacacatgcgctgacagctgtaccgattacgattactctgttccaggccatccgtaagccgttcggccgcccgttcaaagtaacggaaaaaggtggcgatcgttcccaggttcgcgtgcatttgccgactgctatcttttttgcctttgtcacgttatccagtgcggtgagcatcgttctggccgtatacggcctggatgcgcctagcgaactgtcttctcgtgattgcttaaacctgatctggtcggccgtcgccatggtgattgcattcacgtcatttatttgttgcattgaattgcctcgctttggaaaagaggagatgattggtgtcgatttccgcggtcagcttcgttccgcgtcgtcgactcgcccggtacggatcaccggcctgtcgaccgaaaacattacgctggcggcggtaccgtccagttcggatgttaaagatgtgttcgtgccggaggcgggatggatgcgcatcagtccagcggaacatgcgcagaactcgggcaaattcgacatccacccgtctgacgaacagcgtcgctctattctgcgcttattgtttcgcaaggccccggaaaatgtggcggagcaaggtgacttaatgaaaagtatgcgtattcttcttgcacgtgccttcggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z