BBa_K1686046 1 BBa_K1686046 Curdlan synthase gene with codon optimisation for E. coli 2015-09-05T11:00:00Z 2015-09-12T08:06:56Z Curdlan and other bacterial (1→3)-β-D-glucans. M. McIntosh . B. A. Stone . V. A. Stanisich - Appl Microbiol Biotechnol (2005) 68: 163???173; DOI 10.1007/s00253-005-1959-5 (Karnezis et al. 2000, 2003) The curdlan synthase (73 kDa) coded by crdS (1,965 bp) polymerizes UDP glucose into beta 1,3 D Glucan. CrdS shares no homology with the (1→3)- β-glucan synthase-related FSK1 and FSK2 proteins from fungi (e.g. Saccharomyces, Candida, Aspergillus; Dijkgraaf et al. 2001) or the plant callose synthase-related proteins (Li et al. 2003), which are both classified as GT48 glycosyltransferases. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout???Cin disposition). In Agrobacterium, this gene is flancked by two other genes (crdA and crdC) who's corresponding proteins help transport the nascent polymer across the cytoplasmic membrane and into the periplasm. The process of polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane and so is unlikely to involve the periplasmic CrdC, or CrdA directly. false false _2104_ 25369 25369 9 false The gene sequence that was used was found in the publication written below. We then verified that the EcoRI, XbaI, SpeI, PstI sequences were not in the gene which was the case. Finally we optimized the sequence using IDT's optimization software on the internet for E.coli. GenBank : AF057142.2 Publication : ????Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene???? , Stan J.Stasinopoulos & all, 1999 false Jean Descarpentrie annotation2445184 1 Stop codon range2445184 1 1963 1965 annotation2445183 1 Start codon range2445183 1 1 3 BBa_K1686046_sequence 1 atgtatttcagtgctgaaggtgatgtacagagtgtgttatacgtgaacctgacgattgcaatcggtgcaatcctgtttgccctgctggctgatccgcgtaagatggttgatcgcctggcattcagcatcatcatgttgttatctctgggtgtctatattgtctggcgtgccacggacaccttgcctccacctgaactcagcctcgaaacgctgtggtgctatacttatttcaccttcgagctgatcagtgtactgtatgctatgggctcaattctcattttactgcgtcggaccgattggagtgcggtggccgatcagggagaagcctatttagctggcaatccacacgccccgttagtggacgtttttatttgcacctataatgaacctttgaacgtgctcgagaaatcgattattgcggcacaagctatggactatccgcgcttgcgcgtgttcgtgtgtgacgatacccggcgtggggaggtccgcacctactgcgaggcggtgggggtcaactatgtaacccgtcctgataataaacatgctaaagctggcaacttgaataacgctttactgcacaccaacgcgttagaggaagtaagtgactttattatggttctggatgctgactttgcaccacaggcaaacttcctgcgccgcgtcacaggtttgttctcggatcctaaagtcgcggtagtccagactccgcagttttattttaattcagatccaatccagcacaacctgggaatcgataaaagcttcgtagatgatcaacgtgttttttttgacgtgttccagccggcgaaagatgccgtcggttgtgcgttctgcgttgggaccagctttgtggtccgccgggcagccgtaaatgggattgggggctttccttcggacgcgttgtctgaagacatgttgctgacataccgcttaatggaacgtggctacgtgacccgttggctcaatgaaaaactgagtgttggtttaagtgctgaaggagtacctgaatatattacccagcgcactcgttggtgtctgggcactatccagatcggcctgttacgtacgggccccctgtggcgtggcaattttacccttactcagcggctgcattaccttcatggcctgttctgttggctctccaaaccgtttattctgtgtctgctgcttgcgccgagcatctattggctgaccggtgtgagtgcgttgcaggtcgatgaactgatgtttatgaaattaggcttatcgagtctggcactgttttggacctattcgacatggattagcggcaaacgcacccttccgttgttcacggaggttacacatgcgctgacagctgtaccgattacgattactctgttccaggccatccgtaagccgttcggccgcccgttcaaagtaacggaaaaaggtggcgatcgttcccaggttcgcgtgcatttgccgactgctatcttttttgcctttgtcacgttatccagtgcggtgagcatcgttctggccgtatacggcctggatgcgcctagcgaactgtcttctcgtgattgcttaaacctgatctggtcggccgtcgccatggtgattgcattcacgtcatttatttgttgcattgaattgcctcgctttggaaaagaggagatgattggtgtcgatttccgcggtcagcttcgttccgcgtcgtcgactcgcccggtacggatcaccggcctgtcgaccgaaaacattacgctggcggcggtaccgtccagttcggatgttaaagatgtgttcgtgccggaggcgggatggatgcgcatcagtccagcggaacatgcgcagaactcgggcaaattcgacatccacccgtctgacgaacagcgtcgctctattctgcgcttattgtttcgcaaggccccggaaaatgtggcggagcaaggtgacttaatgaaaagtatgcgtattcttcttgcacgtgccttcggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z