BBa_K1687000 1 BBa_K1687000 E.C. 1.7.7.2 Generator 2015-09-15T11:00:00Z 2015-09-16T03:58:18Z 1000000000000 00000000000000000000000 false false _2105_ 24745 24745 9 true 00000000000000 false 2014&2015 Shenzhen Middle School annotation2458757 1 EC 1.7.7.2 generator range2458757 1 1 1953 BBa_K1687002 1 BBa_K1687002 EC 1.7.7.2 + E0240 2015-09-16T11:00:00Z 2015-09-17T11:00:37Z E0240 comes from the iGEM Distribution while EC 1.7.7.2 is from BBa_K1687000 A composite part combining a ferredoxin-nitrate reductase generator (without RBS) and an GFP with RBS and terminator. false false _2105_ 24745 19427 9 false None. false Yumeng Li component2465735 1 BBa_K1687000 component2465746 1 BBa_E0240 annotation2465746 1 BBa_E0240 range2465746 1 1968 2843 annotation2465735 1 BBa_K1687000 range2465735 1 1 1959 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_E0240 1 GFP report GFP generator 2004-10-17T11:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 B0032.E0040.B0015 false true _11_1_ 0 61 7 In stock false true Jennifer Braff component1249231 1 BBa_B0012 component1249213 1 BBa_B0032 component1249221 1 BBa_B0010 component1249216 1 BBa_E0040 annotation1249231 1 BBa_B0012 range1249231 1 836 876 annotation1249221 1 BBa_B0010 range1249221 1 748 827 annotation1249216 1 BBa_E0040 range1249216 1 20 739 annotation1249213 1 BBa_B0032 range1249213 1 1 13 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1687000_sequence 1 ttaaactggtgtaatattaacggcgcaggcttttaattccggttgtttagaaatagggcaagcagtggcatgggtcaaactattaacttcagcattatcgtcccacaaaaaaccccagtgcatgggcataaaaaccgtaccgggggcaatggctctagtaactagaacttctagctgagcagaaccccgtcgagatttaatcgcaactaaatcctgacttttaatacccaatttttctgcgtccttgggatgcatttccaatataggtttgggatgcatttttttgattttctcaatgtggccagtgcgggtttgggtatgccaatgaccataaagacgaccattggtgaggacaaaaggatagtttggatcgaccggctcagctaatccccgactgtggtctaagcaaaagtttgcccgaccattttctgtgcaaaatttatagtcggtgtaaagtcttttagttgataaaagttgactttcttgatctgattgacgacagggccattgcagcggccccagcttttgcaacttttcataactgacaccagattggtcacataatctttcagcagttagttgcacatattcccgatgtactgcggctgaatcaacaaaattaaattgattttcaaaacctaatctacgtcctacctcagcaaaaatttcccagtcgggtcttgcttccccaggaggagagcgaaacgcaggagaaagggtaactctccgttctgaattggtcattgtgccagttttttcgctccattgagctgctggcaataacaaatgggcgtattctgccgtttcagtgggggaataggcatcctgatgaatcgtaaaagttgattgttgcagagcctttttaaccctttctaaatcaggcatactaacaacagggttagtcgccacaatccataggaaaccaacctgttgattttccaatcccatgaacatttcccaggctgataaacctggttctggattaatacggccaggggaaatctgccaaagttgttctacctcttggcggtgttgaggatttttaattgaacgatagccaggcaacagatgggacaatccccctgcttcccgaccccccatggcgttgggctgaccagtgagggaaaaaggtccactgcctggtttcccaatctgaccagtgagtaaatggagattaattaaacaacgacctttagctgtgccttgaaaagattgattcattcccatggaccaaagggaaagcactgttttggcattgccccaatagtgagcggcggtttctaattgttctaaactaatgccacaaatttgggtaactttttccgggggataatgatcaataacttgacaatactgctcaaaatcttgggtgtgattatcaataaatttttgatcaattaaattccacttttttagcaaataggcaatgccatggagtaaagtaatgtcactgccaggattgatcgctaaatgtaagtccgcaacttctgcggtggcagtacaacgggggtccaccacaattaatttaacgtggggattttgtttgtggtgtttgcgtaaacgattaaaaataatgggatggcagtccgctgtattagtaccaattaaaaaagcgcaatcggttatttctaagtcttcataacagcaaggcggaccatcggaaccaaagcttaggctataggcagacacagccgaagacatgcaaagacgagaattggtatcaaaattattggtacccaaacaacctttaaataatttttgggccacgtagtaatcctctgtctgcatttgcccagaggcgtacatacataaaccacttacccccaaagtttgtttaacttgctgaattttatcaacaacaatttctagggctgcttcccaactaatttgttgccacggctgatctaaacttgaccgaaacatggggtaaagtaatcgctgtttatccatggattccat BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1687002_sequence 1 ttaaactggtgtaatattaacggcgcaggcttttaattccggttgtttagaaatagggcaagcagtggcatgggtcaaactattaacttcagcattatcgtcccacaaaaaaccccagtgcatgggcataaaaaccgtaccgggggcaatggctctagtaactagaacttctagctgagcagaaccccgtcgagatttaatcgcaactaaatcctgacttttaatacccaatttttctgcgtccttgggatgcatttccaatataggtttgggatgcatttttttgattttctcaatgtggccagtgcgggtttgggtatgccaatgaccataaagacgaccattggtgaggacaaaaggatagtttggatcgaccggctcagctaatccccgactgtggtctaagcaaaagtttgcccgaccattttctgtgcaaaatttatagtcggtgtaaagtcttttagttgataaaagttgactttcttgatctgattgacgacagggccattgcagcggccccagcttttgcaacttttcataactgacaccagattggtcacataatctttcagcagttagttgcacatattcccgatgtactgcggctgaatcaacaaaattaaattgattttcaaaacctaatctacgtcctacctcagcaaaaatttcccagtcgggtcttgcttccccaggaggagagcgaaacgcaggagaaagggtaactctccgttctgaattggtcattgtgccagttttttcgctccattgagctgctggcaataacaaatgggcgtattctgccgtttcagtgggggaataggcatcctgatgaatcgtaaaagttgattgttgcagagcctttttaaccctttctaaatcaggcatactaacaacagggttagtcgccacaatccataggaaaccaacctgttgattttccaatcccatgaacatttcccaggctgataaacctggttctggattaatacggccaggggaaatctgccaaagttgttctacctcttggcggtgttgaggatttttaattgaacgatagccaggcaacagatgggacaatccccctgcttcccgaccccccatggcgttgggctgaccagtgagggaaaaaggtccactgcctggtttcccaatctgaccagtgagtaaatggagattaattaaacaacgacctttagctgtgccttgaaaagattgattcattcccatggaccaaagggaaagcactgttttggcattgccccaatagtgagcggcggtttctaattgttctaaactaatgccacaaatttgggtaactttttccgggggataatgatcaataacttgacaatactgctcaaaatcttgggtgtgattatcaataaatttttgatcaattaaattccacttttttagcaaataggcaatgccatggagtaaagtaatgtcactgccaggattgatcgctaaatgtaagtccgcaacttctgcggtggcagtacaacgggggtccaccacaattaatttaacgtggggattttgtttgtggtgtttgcgtaaacgattaaaaataatgggatggcagtccgctgtattagtaccaattaaaaaagcgcaatcggttatttctaagtcttcataacagcaaggcggaccatcggaaccaaagcttaggctataggcagacacagccgaagacatgcaaagacgagaattggtatcaaaattattggtacccaaacaacctttaaataatttttgggccacgtagtaatcctctgtctgcatttgcccagaggcgtacatacataaaccacttacccccaaagtttgtttaacttgctgaattttatcaacaacaatttctagggctgcttcccaactaatttgttgccacggctgatctaaacttgaccgaaacatggggtaaagtaatcgctgtttatccatggattccattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_E0240_sequence 1 tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z