BBa_K1331001 1 BBa_K1331001 Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6 2014-09-24T11:00:00Z 2015-05-08T01:09:56Z <i>Pseudomonas aeruginosa</i> RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in <i>Pseudomonas aeruginosa</i>. false false _1706_ 0 20153 9 In stock false A mutation has been done to remove the PstI restriction site contained in the coding sequence. false Tianyi Huang annotation2386609 1 rhlA range2386609 1 1 888 annotation2427978 1 noncoding sequence range2427978 1 889 947 BBa_K1688002 1 BBa_K1688002 RhlA Gene 2015-09-14T11:00:00Z 2015-09-15T11:14:18Z RhlA was lifted from 2015 DNA distribution kit and assembled with RBS The enzymes responsible for producing the mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The mono- rhamnolipids consist of a hydrophobic tail and a hydrophilic head. The RhlA gene codes for the fatty acid tail and it has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick from team Nankai BBa_K1331001 consists of gene RhlA whereas the biobrick BBa_K1688002 consist of a ribosomal binding site and a gene coding RhlA. false false _2106_ 26592 26592 9 Not in stock false no special design were considered false Biosurfers component2457147 1 BBa_B0034 component2457150 1 BBa_K1331001 annotation2457147 1 BBa_B0034 range2457147 1 1 12 annotation2457150 1 BBa_K1331001 range2457150 1 19 965 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1688002_sequence 1 aaagaggagaaatactagatgcggcgcgaaagtctgttggtatcggtttgcaagggcctgcgggtacatgtcgagcgcgttgggcaggatcccgggcgcagcacggtgatgctggtcaacggcgcgatggcgaccaccgcctcgttcgcccggacctgcaagtgcctggccgaacatttcaacgtggtgctgttcgacctgcccttcgccgggcagtcgcgtcagcacaacccgcagcgcgggttgatcaccaaggacgacgaggtggaaatcctcctggcgctgatcgagcgcttcgaggtcaatcacctggtctccgcgtcctggggcggtatctccacgctgctggcgctgtcgcgcaatccgcgcggtatccgcagctcggtggtgatggcattcgcccctggactgaaccaggcgatgctcgactacgtcgggcgggcgcaggcgctgatcgagctggacgacaagtcggcgatcggccatctgctcaacgagaccgtcggcaaatacctgccgccgcgcctgaaagccagcaaccatcagcacatggcttcgctggccaccggcgaatacgagcaggcgcgctttcacatcgaccaggtgctggcgctcaacgatcggggctacctggcttgcctggagcggatccagagccacgtgcatttcatcaacggcagctgggacgaatacaccaccgccgaggacgcccgccagttccgcgactacctgccgcactgtagtttctcgcgggtggagggcaccgggcatttcctcgacctggagtccaagctggccgcggtacgcgtgcaccgcgccctgctcgagcacctgctgaagcaaccggagccgcagcgggcggaacgcgcggcgggattccacgagatggccatcggctacgcctgaacccttgacctgcgaagacccggcctggccgggctttgcggttgcataacgcacggagt BBa_K1331001_sequence 1 atgcggcgcgaaagtctgttggtatcggtttgcaagggcctgcgggtacatgtcgagcgcgttgggcaggatcccgggcgcagcacggtgatgctggtcaacggcgcgatggcgaccaccgcctcgttcgcccggacctgcaagtgcctggccgaacatttcaacgtggtgctgttcgacctgcccttcgccgggcagtcgcgtcagcacaacccgcagcgcgggttgatcaccaaggacgacgaggtggaaatcctcctggcgctgatcgagcgcttcgaggtcaatcacctggtctccgcgtcctggggcggtatctccacgctgctggcgctgtcgcgcaatccgcgcggtatccgcagctcggtggtgatggcattcgcccctggactgaaccaggcgatgctcgactacgtcgggcgggcgcaggcgctgatcgagctggacgacaagtcggcgatcggccatctgctcaacgagaccgtcggcaaatacctgccgccgcgcctgaaagccagcaaccatcagcacatggcttcgctggccaccggcgaatacgagcaggcgcgctttcacatcgaccaggtgctggcgctcaacgatcggggctacctggcttgcctggagcggatccagagccacgtgcatttcatcaacggcagctgggacgaatacaccaccgccgaggacgcccgccagttccgcgactacctgccgcactgtagtttctcgcgggtggagggcaccgggcatttcctcgacctggagtccaagctggccgcggtacgcgtgcaccgcgccctgctcgagcacctgctgaagcaaccggagccgcagcgggcggaacgcgcggcgggattccacgagatggccatcggctacgcctgaacccttgacctgcgaagacccggcctggccgggctttgcggttgcataacgcacggagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z