BBa_K1688003 1 BBa_K1688003 RhlB Gene 2015-09-14T11:00:00Z 2015-09-18T11:16:43Z The RhlA gene (BBa_K1331004) and RBS ((BBa_B0034) was lifted from 2015 DNA Distribution Kit. The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB.. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene (BBa_K1688002) codes for the fatty acid tail and the RhlB connects the tail to a rhamnose molecule. The RhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick BBa_K1688003 is an assembly of RBS with BBa_K1331004 false false _2106_ 26589 26592 9 false no specific design false Biosurfers component2457064 1 BBa_K1331004 component2457060 1 BBa_B0034 annotation2457060 1 BBa_B0034 range2457060 1 1 12 annotation2457064 1 BBa_K1331004 range2457064 1 21 1327 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1331004 1 BBa_K1331004 Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6 2014-09-24T11:00:00Z 2015-05-08T01:09:56Z Pseudomonas aeruginosa SQ6 RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence. false false _1706_ 0 20153 9 In stock false Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence. false Tianyi Huang annotation2434208 1 noncoding sequence range2434208 1 1 6 annotation2434210 1 noncoding sequence range2434210 1 1288 1307 annotation2434209 1 RhlB range2434209 1 7 1287 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1331004_sequence 1 agccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcctggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccggaccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggaaacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccgggaaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctccagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttgccgaaccacgtgctccagcgcgcctacgcgccactgggagccttgctgccatcgtgcgccgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgcaggtgctgctgccctgtgcccacgaccagttcgacaatgctgaacggctggtccggctcggctgcgggatgcgcctgggcgtgccgttgcgcgagcaggagttgcgcggggcgctgtggcgcttgctcgaggacccggccatggcggcggcctgtcggcgtttcatggaattgtcacaaccgcacagtatcgcttgcggtaaagcggcccaggtggtcgaacgttgtcatagggagggggatgctcgatggctgaaggctgcgtcctgaacggtgctggcataacagat BBa_K1688003_sequence 1 aaagaggagaaatactagagagccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcctggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccggaccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggaaacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccgggaaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctccagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttgccgaaccacgtgctccagcgcgcctacgcgccactgggagccttgctgccatcgtgcgccgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgcaggtgctgctgccctgtgcccacgaccagttcgacaatgctgaacggctggtccggctcggctgcgggatgcgcctgggcgtgccgttgcgcgagcaggagttgcgcggggcgctgtggcgcttgctcgaggacccggccatggcggcggcctgtcggcgtttcatggaattgtcacaaccgcacagtatcgcttgcggtaaagcggcccaggtggtcgaacgttgtcatagggagggggatgctcgatggctgaaggctgcgtcctgaacggtgctggcataacagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z