BBa_K1688003
1
BBa_K1688003
RhlB Gene
2015-09-14T11:00:00Z
2015-09-18T11:16:43Z
The RhlA gene (BBa_K1331004) and RBS ((BBa_B0034) was lifted from 2015 DNA Distribution Kit.
The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB.. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene (BBa_K1688002) codes for the fatty acid tail and the RhlB connects the tail to a rhamnose molecule. The RhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick BBa_K1688003 is an assembly of RBS with BBa_K1331004
false
false
_2106_
26589
26592
9
false
no specific design
false
Biosurfers
component2457064
1
BBa_K1331004
component2457060
1
BBa_B0034
annotation2457060
1
BBa_B0034
range2457060
1
1
12
annotation2457064
1
BBa_K1331004
range2457064
1
21
1327
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1331004
1
BBa_K1331004
Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
2014-09-24T11:00:00Z
2015-05-08T01:09:56Z
Pseudomonas aeruginosa SQ6
RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
false
false
_1706_
0
20153
9
In stock
false
Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
false
Tianyi Huang
annotation2434208
1
noncoding sequence
range2434208
1
1
6
annotation2434210
1
noncoding sequence
range2434210
1
1288
1307
annotation2434209
1
RhlB
range2434209
1
7
1287
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1331004_sequence
1
agccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcctggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccggaccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggaaacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccgggaaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctccagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttgccgaaccacgtgctccagcgcgcctacgcgccactgggagccttgctgccatcgtgcgccgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgcaggtgctgctgccctgtgcccacgaccagttcgacaatgctgaacggctggtccggctcggctgcgggatgcgcctgggcgtgccgttgcgcgagcaggagttgcgcggggcgctgtggcgcttgctcgaggacccggccatggcggcggcctgtcggcgtttcatggaattgtcacaaccgcacagtatcgcttgcggtaaagcggcccaggtggtcgaacgttgtcatagggagggggatgctcgatggctgaaggctgcgtcctgaacggtgctggcataacagat
BBa_K1688003_sequence
1
aaagaggagaaatactagagagccccatgcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcctggcccggaccctgaaattgcgcgggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctgagcgacgaactgacctaccgccggaccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggcaagccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcgctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgaccttgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgctctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctggaaacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcgcgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccgggaaccccgctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacacctccagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggccaggaaccgctgcgcggcttgccgaaccacgtgctccagcgcgcctacgcgccactgggagccttgctgccatcgtgcgccgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgcaggtgctgctgccctgtgcccacgaccagttcgacaatgctgaacggctggtccggctcggctgcgggatgcgcctgggcgtgccgttgcgcgagcaggagttgcgcggggcgctgtggcgcttgctcgaggacccggccatggcggcggcctgtcggcgtttcatggaattgtcacaaccgcacagtatcgcttgcggtaaagcggcccaggtggtcgaacgttgtcatagggagggggatgctcgatggctgaaggctgcgtcctgaacggtgctggcataacagat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z